hcg detection reagent, test paper and using method thereof
A kit and chromatography test paper technology, applied in the field of immune detection, can solve the problems of complicated detection process, large influence of environmental temperature, narrow detection range, etc., and achieve the effect of accurate and reliable detection results and improved reliability.
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Embodiment 1
[0066] The preparation of embodiment 1 reaction reagent
[0067] Dissolve FITC in 10mM PBS solution at a certain concentration, take a certain amount of mouse anti-β-HCG monoclonal antibody 4 (Sichuan Mike Biological New Material Technology Co., Ltd., XCL1401) into the dialysis bag, put the dialysis bag into the FITC solution Place it on a magnetic stirrer, and stir and dialyze overnight in the dark. After dialysis, take out FITC-mouse anti-β-HCG monoclonal antibody 4 and store at 4°C.
[0068] Take an appropriate volume of fluorescent microspheres (JSR, F7655S) solution and add EDC (0.1mg / 1mg fluorescent microspheres) activator to activate for 30min, then add FITC-mouse anti-β-HCG single Antibody 4 was cloned, stirred and reacted for 1 hour at a constant speed, then a blocking agent was added, and the reaction was terminated for 30 minutes, and finally a complex of FITC~mouse anti-β-HCG monoclonal antibody 4~fluorescent microspheres was obtained.
[0069] Take an appropriat...
Embodiment 2
[0071] Embodiment 2 detects the preparation of test paper
[0072] Dilute the mouse anti-β-HCG monoclonal antibody 3 (Sichuan Mike Biological New Material Technology Co., Ltd., XCL1400) to 2 mg / mL with coating buffer; Co., Ltd., XCL1510) was diluted to 2 mg / mL, goat anti-rabbit IgG was diluted to 1 mg / mL, and anti-FITC monoclonal antibody was mixed with goat anti-rabbit IgG at a ratio of 1:1. Draw the mouse anti-β-HCG monoclonal antibody 3 on the detection line with a film drawer, put the anti-FITC monoclonal antibody and goat anti-rabbit IgG quality control line (spray volume: 1 μL / cm), and dry at 55°C for 3 days .
Embodiment 3
[0073] Embodiment 3 detection method
[0074] Take 20 μL sample (or quality control product) and add it to 280 μL / tube of reaction solution, mix well, add 90 μL of the mixed solution into the sample hole of the test card, and after chromatography at room temperature for 15 minutes, insert the test card into the socket of the immunofluorescence detector. In the loading hole, the instrument will automatically scan the test card and calculate the results.
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