Adverse resistance related protein IbBBX24 and coding gene and application thereof

An ibbbx24, coding technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve inter-species and intra-species hybrid incompatibility, limit the utilization of sweet potato breeding resources and the free combination of parents, and it is difficult to breed high-quality, New varieties of sweet potato with high yield and resistance to vine-cutting disease, etc.

Inactive Publication Date: 2019-02-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Sweet potato is a vegetatively propagated crop with incompatibility between interspecific and intraspecific hybrids, which severely limits the resource utilization and free assemblage of parents in sweet p

Method used

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  • Adverse resistance related protein IbBBX24 and coding gene and application thereof
  • Adverse resistance related protein IbBBX24 and coding gene and application thereof
  • Adverse resistance related protein IbBBX24 and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1, the acquisition of IbBBX24 gene

[0086] The steps of obtaining the IbBBX24 gene are as follows:

[0087] 1. Obtaining the template

[0088] The total RNA of the young leaves of the sweet potato salt-tolerant mutant ND98 was extracted with a plant total RNA extraction kit, and the total RNA was extracted with PrimeScript TM 1st Strand cDNA Synthesis Kit reverse transcribes the first strand cDNA.

[0089] 2. Construct the cDNA-AFLP subtraction library, and obtain the EST sequence shown in sequence 4 in the sequence listing. According to the nucleotide sequence of EST sequence, primers GSP-1 and GSP-2 were designed and synthesized artificially.

[0090] 3. After completing step 2, use the cDNA obtained in step 1 as a template, use the GSP-1 and GSP-2 synthesized in step 3 as primers, and use the RACE method to amplify a 3'-RACE fragment of about 700 bp, and convert the 3'- The RACE fragment was connected with the cloning vector pMD19-T to obtain recombi...

Embodiment 2

[0099] Embodiment 2, the application of IbBBX24 protein in regulating the stress resistance of sweet potato

[0100] 1. Construction of recombinant plasmids

[0101] A, construction of recombinant plasmid pCB-IbBBX24

[0102] 1. Artificially synthesize the double-stranded DNA molecule shown in Sequence 1 of the Sequence Listing. Using the double-stranded DNA molecule as a template, OE-F-XbaI: 5′-GC TCTAGA ATGAAGATTCAGTGTGATGTGTGT-3' (underlined is the recognition sequence of restriction endonuclease XbaI) and OE-R-SacI: 5'-C GAGCTC TCAACCGAGATCAGGGACAGT-3' (the underline is the recognition sequence of restriction endonuclease SacI) is used as a primer for PCR amplification to obtain a double-stranded DNA molecule containing restriction endonuclease XbaI at the N-terminus and restriction endonuclease SacI at the C-terminus.

[0103] 2. Ligate the double-stranded DNA molecule containing the restriction endonuclease XbaI at the N-terminal and the restriction endonuclease Sac...

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Abstract

The invention discloses an adverse resistance related protein IbBBX24 and a coding gene and application thereof. The protein IbBBX24 is the protein shown in following (1) or (2) or (3), specifically,(1) the amino acid sequence of the protein is shown as the sequence 2 in a sequence table; (2) the N terminal or/and C terminal of the protein shown by the sequence 2 in the sequence table is connected with a label to obtain a fusion protein; and (3) the protein related to plant adverse resistance is obtained by replacing and/or missing and/or adding of one or more amino acid residues into the protein shown in (1) or (2). Through experiments, it is shown that the adverse resistance of ipomoea batatas (L .) Lam . can be improved by overexpressing an IbBBX24 gene in the ipomoea batatas (L .) Lam., and the adverse resistance of the ipomoea batatas (L .) Lam . can be lowered by interfering in the IbBBX24 gene; and the adverse resistance can be salt resistance and/or drought resistance and/oroxidation resistance and/or disease resistance. Therefore, the adverse resistance related protein IbBBX24 and the coding gene thereof have important theoretical significance and practical value in regulating the plant adverse resistance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an anti-stress related protein IbBBX24 and its coding gene and application. Background technique [0002] Sweet potato (Ipomoea batatas (L.) Lam.) is an important food, feed and industrial raw material crop, and as a new energy plant in today's world, its status is particularly important. my country is the largest sweet potato producer in the world, with an annual planting area of ​​3.387 million hm 2 , accounting for 42.2% of the world's total planting area, and its annual output accounts for 67.94% of the world's total output. With the continuous reduction of cultivated land area and the continuous increase of energy pressure, most of the sweet potatoes are planted in the southeast coastal slopes and the vast arid areas in the west. Further development brought certain difficulties. In addition, sweet potato fungal diseases seriously affect sweet potato production, es...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82
CPCC07K14/415C12N15/8271C12N15/8273C12N15/8282
Inventor 何绍贞刘庆昌翟红张欢赵宁李仁崑
Owner CHINA AGRI UNIV
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