Application of strigolactone in promoting oil accumulation of single needle algae

A technology for accumulation of strigolactone and oil, applied in the field of bioengineering, can solve problems such as low oil yield of microalgae, and achieve the effects of high-quality culture, fast and high-density growth, and promotion of oil accumulation.

Active Publication Date: 2019-02-12
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the limitations of existing te

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  • Application of strigolactone in promoting oil accumulation of single needle algae

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Embodiment 1

[0027] The application of strigolactone in promoting the accumulation of monopodella oil, the application method specifically includes the following steps:

[0028] (1) Using BG-11 medium as the basic medium, glucose as the carbon source, adding 10g / L to the BG-11 medium, heterotrophically cultivating the monospina and the algae species of monospina under constant temperature conditions of 25°C for Monoraphidium sp. QLY-1, collect the cells of Monopodella after it grows to the late logarithmic phase;

[0029] (2) Prepare induction medium: use BG-11 medium as the basal medium for Monopodella oleaginum, adjust the pH value to 7 with 1mol / L sodium hydroxide solution, and sterilize at 121°C and 0.1MPa for 20min ;

[0030] (3) Induction of Monopodella oleaginum: Insert the Monopodella cells collected in step (1) into the sterilized medium in step (2), the concentration of Monopodella cells is 0.7g / L, and then add The strigolactone SLs mother solution prepared with acetone, where...

Embodiment 2

[0034] The application of strigolactone in promoting the accumulation of monopodella oil, the application method specifically includes the following steps

[0035] (1) Using BG-11 medium as the basic medium, glucose as the carbon source, adding 8g / L to the BG-11 medium, heterotrophically cultivating the monospina and the algae species of monospina under constant temperature conditions of 23°C for Monoraphidium sp. QLY-1, collect the cells of Monopodella after it grows to the late logarithmic phase;

[0036](2) Prepare induction medium: use BG-11 medium as the basal medium for Monopodella oleaginum, adjust the pH value to 6.8 with 1mol / L hydrochloric acid, and sterilize at 121°C and 0.1MPa for 20 minutes;

[0037] (3) Induction of Monopodella oleaginum: Insert the Monopodella cells collected in step (1) into the sterilized medium in step (2), the concentration of Monopodella cells is 0.7g / L, and then add The strigolactone SLs mother solution prepared with acetone, wherein the...

Embodiment 3

[0041] The application of strigolactone in promoting the accumulation of monopodella oil, the application method specifically includes the following steps

[0042] (1) Using BG-11 medium as the basic medium, glucose as the carbon source, adding 11g / L to the BG-11 medium, heterotrophically cultivating the monospina and the algae species of monospina under constant temperature conditions of 26°C for Monoraphidium sp. QLY-1, collect the cells of Monopodella after it grows to the late logarithmic phase;

[0043] (2) Prepare induction medium: use BG-11 medium as the basal medium for Monopodella oleaginum, adjust the pH value to 6.9 with 1mol / L hydrochloric acid, and sterilize at 121°C and 0.1MPa for 20min;

[0044] (3) Induction of Monopodella oleaginum: Insert the Monopodella cells collected in step (1) into the sterilized medium in step (2), the concentration of Monopodella cells is 0.7g / L, and then add The strigolactone SLs mother solution prepared with acetone, wherein the st...

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Abstract

The invention discloses application of strigolactone in promoting oil accumulation in single needle algae, and realizes the rapid high-density growth and high-quality culture of the single needle algae through two-stage cultivation of the single needle algae. The mother liquor of strigolactone is added into induced algae liquid, a medium is cultured under light, and the oil is extracted to preparebiodiesel; the addition of exogenous strigolactone can effectively promote the oil accumulation in cells of the heterotrophic single needle algae; the application of the strigolactone is simple and easy to implement, can greatly increase the oil content, thereby improving the efficiency of producing biodiesel by the single needle algae.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to the application of strigolactone in promoting the accumulation of oil in monospina. Background technique [0002] In recent years, due to the extensive application of fossil fuels, the depletion of fossil fuels has resulted. As an alternative fuel to fossil fuels, biodiesel has become the fastest growing and most widely used environmentally friendly renewable energy in the world. Biodiesel, also known as biodiesel, is a kind of raw material prepared from unprocessed or used vegetable oil, microbial oil, animal oil, waste cooking oil, etc. through transesterification or thermochemical process, which is considered environmentally friendly. biomass fuel. Compared with conventional petroleum, it has the advantages of environmental protection, renewable, degradable, good stability, high cetane number, easy storage, and high safety performance. [0003] Microalgae is an auto...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/12C12P7/64C12R1/89
CPCC12N1/12C12N1/38C12P7/6463Y02E50/10
Inventor 余旭亚宋雪婷赵永腾
Owner KUNMING UNIV OF SCI & TECH
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