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Thermal-stability Newcastle disease virus attenuated vaccine candidate strain

A technology of Newcastle disease virus and attenuated vaccine is applied in the field of candidate strains of attenuated vaccine of Newcastle disease virus to achieve the effect of good immunogenicity of the virus strain and high level of antibody production

Inactive Publication Date: 2019-02-12
YANGZHOU UNIV
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Problems solved by technology

Early research results showed that among the strains of different virulence types of NDV, there are strains with higher temperature tolerance, mainly showing that they still have the ability to infect the host after being treated at 56°C for 30 minutes, or although they lose the ability to Infectious, but still active in HA

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  • Thermal-stability Newcastle disease virus attenuated vaccine candidate strain
  • Thermal-stability Newcastle disease virus attenuated vaccine candidate strain
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Embodiment Construction

[0029] Biomaterials involved in the present invention:

[0030] The LaSota strain was purchased from China Veterinary Drug Administration (CVCCNo: AV615),

[0031] ZJ1 strain (Liu X F, Wan H Q, Ni X X, et al. Pathotypical and genotypical characterization of strain of Newcastle disease virus isolated from out breaks in chicken and goose flocks in some regions of China during 1985-2001. ArchVirol, 2003, 148(7): 1387-1403.). NDV / HR09 strain (Cao Y, Liu Q, Zhang X, Hu H, WuY. 2017. Complete genome sequence of heat resistant Newcastle disease virus strain HR09. Genome Announc 5:e01149-17.).

[0032] Step 1: Identification of heat-resistant strain NDV / HR09

[0033] The heat resistance of the strains isolated and stored in the Key Laboratory of Livestock and Poultry Infectious Diseases of the Ministry of Agriculture of Yangzhou University and the LaSota strain were evaluated for heat resistance (Table 1). The HA activity after 56 °C treatment for 30 minutes was used as the judgment...

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Abstract

The invention relates to a veterinarian virus molecular biology technology and an immunology technology and discloses a thermal-stability Newcastle disease virus novel (gene VIII type) attenuated vaccine candidate strain NDV / rAHR09 and a construction method thereof. The preservation number of the thermal-stability Newcastle disease virus attenuated vaccine candidate strain is CCTCC (China Center for Type Culture Collection) NO: V201739. A research shows that the NDV / rAHR09 strain has good thermal stability and still keeps blood coagulation activity and infection capability at 56 DEG C after 60min; an immunity test shows that the thermal-stability Newcastle disease virus attenuated vaccine candidate strain has a good immune protection effect on a Newcastle disease virulent strain; the strain, a common vaccine strain LaSota and a heat-resisting V4 strain are inoculated into 1-day-age SPF chickens respectively and the antibody valence of the chickens is detected every week; a test resultproves that the antibody valence of the chickens immunized by the virulent strain is remarkably higher than that of the LaSota strain and the V4 strain and the toxin-invading protection rate on prevalent virulent strains is 100 percent.

Description

technical field [0001] The invention relates to the fields of veterinary virus molecular biology technology and immunology, in particular to a new genotype, heat-resistant Newcastle disease virus attenuated vaccine candidate strain. Background technique [0002] Newcastle disease virus (Newcastle disease virus, NDV) is very harmful to poultry farming industry in the world, it can infect many kinds of poultry, often causes poultry industry to produce huge economic loss [Alexander, D.J.2001.GordonMemorial Lecture.Newcastle disease.Br Poult Sci 42:5-22.]. The virus belongs to the members of Paramyxoviridae, Paramyxovirinae, and Avulavirus in taxonomy [Mayo, M.A. 2002. Virus taxonomy-Houston 2002. Arch Virol 147:1071-1076.]. Newcastle disease virus has an envelope, and its genome is a single-strand, negative-strand, non-segmented RNA virus. The genome structure pattern is: 3′-NP-P-M-F-HN-L-5′, which encodes six structural proteins in sequence: nuclear Capsid protein (NP), phos...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/04C12N15/40C12N15/63
CPCC07K14/005C12N7/00C12N15/63C12N2760/18121C12N2760/18122
Inventor 曹永忠吴艳涛张小荣刘倩
Owner YANGZHOU UNIV
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