Method for simultaneous detection of 3 emulsifiers in compound emulsifiers

Abstract

The invention relates to a method for simultaneously detecting three emulsifiers in a composite emulsifier. The three emulsifiers in the composite emulsifier are respectively Tween 80, glyceryl monostearate and sucrose fatty acid ester, comprising the steps of: (1), prepare a series of mixed standard working solutions and sample solutions to be tested; (2), under preset chromatographic conditions, inject the series of mixed standard working solutions in step (1) into the high performance liquid chromatograph, and carry out Gradient elution and detection, qualitative with retention time, draw the standard curve of corresponding emulsifier according to the corresponding relationship between the size of the peak area measured by each emulsifier and the corresponding concentration; (3), preset chromatographic conditions in step (2) Next, inject the sample solution to be detected in the step (1) into the high performance liquid chromatograph for sample analysis, qualitatively with the retention time, according to the standard curve of each emulsifier in the step (2), quantitatively calculate the three kinds of emulsifiers in the sample solution Concentration and amount of emulsifier.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to high-performance liquid chromatography analysis and detection, in particular to a method for simultaneously detecting three emulsifiers in a composite emulsifier. Background technique [0002] An emulsifier is a surfactant with a hydrophilic group and an lipophilic group, which can dissolve two phases that are originally immiscible to form a stable and uniform emulsion. After adding an emulsifier to the feed, the oil in the feed can dissolve with water to form fine oil droplets, which increases the action area of ​​lipase and greatly enhances the digestion and absorption capacity of oil. Usually commercial emulsifier products are not composed of a single emulsifier. For better emulsifying performance, several emulsifiers are composed of a compound emulsifier according to an appropriate ratio. Composite emulsifier is an emulsifier composed of more than two kinds of...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many detection methods for emulsifiers, the most common ones are high performance liquid chromatography, capillary gas chromatography, violet chemical titration, gel chromatography, etc., but most of these detection methods are for the determination of a single emulsifier, and all It is some unconventional detectors and chromatographic columns, such as ELSD detectors, gel chromatographic columns, etc., and the detection sensitivity needs to be improved

Therefore, when it is necessary to detect the emulsifier components in the compound emulsifier, it is necessary to use its own pretreatment technology and analysis method, and the detection is time-consuming, laborious, and expensive.

There are also reports of using high performance liquid chromatography tandem mass spectrometry to detect various emulsifiers, but this kind of analytical instrument is expensive and is not easy to be widely promoted in my country's feed industry

[0004] In view of the situation that the pre-sample pretreatment steps are complicated, the analysis period is long, the work efficiency is low, the detection cost is high, and the equipment is not popular when detecting multiple emulsifier substances at the same time. The equipment and facilities are not popular and the detection cost is high. The present invention provides a detection method for simultaneous detection of three emulsifiers in the compound emulsifier

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