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A kind of detection method of glycine betaine in aquatic products

A technology of glycine betaine and detection method, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity, high detection difficulty, poor detection effect, etc., and achieves the effect of high sensitivity and suitable for popularization and application

Active Publication Date: 2021-11-02
宁波市产品食品质量检验研究院(宁波市纤维检验所)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the detection methods for glycine betaine content use colorimetric method, although the method is simple to operate, but it suffers from serious interference, low sensitivity and poor detection effect
In addition, aquatic products (including crabs, shrimps, shellfish, etc.) are not like goji berries and sugar beets, which are single-matrix products with high purity and high concentration of betaine. Good method for detection of glycine betaine in matrix

Method used

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  • A kind of detection method of glycine betaine in aquatic products
  • A kind of detection method of glycine betaine in aquatic products
  • A kind of detection method of glycine betaine in aquatic products

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Experimental program
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Embodiment 1

[0044] Embodiment 1: a kind of detection method of glycine betaine in aquatic products comprises the following steps:

[0045] 1) Weigh the glycine betaine standard substance and dilute it step by step with acetonitrile solution, and dilute it into 6 different concentrations (10μg / mL, 20μg / mL, 30μg / mL, 60μg / mL, 100μg / mL, 200μg / mL concentrations) Standard solution, these 6 kinds of standard solutions of different concentrations are carried out liquid chromatography detection with high performance liquid chromatography (waters e2695) respectively, measure the chromatographic peak area value corresponding to the standard solution of different concentrations, obtain standard chromatogram, wherein chromatogram The conditions are:

[0046] a) Chromatographic column: a BEH HILIC chromatographic column with a particle size of 2.5 μm and a specification of 4.6×150 mm;

[0047] b) Column temperature: 25°C;

[0048] c) mobile phase: water: acetonitrile (V: V) = 20: 80 mixed solution, i...

Embodiment 2

[0064] Embodiment 2: Other contents are all identical with embodiment 1, and difference is that the chromatographic condition in step 1) and step 4) is:

[0065] a) Chromatographic column: a BEH HILIC chromatographic column with a particle size of 2.5 μm and a specification of 4.6×150 mm;

[0066] b) Column temperature: 20°C;

[0067] c) mobile phase: water: acetonitrile (V: V) = 40: 60 mixed solution, isocratic elution;

[0068] d) Mobile phase flow rate: 0.6mL / min;

[0069] e) Injection volume: 5 μL;

[0070] f) Detector type: fluorescence detector;

[0071] g) Detection wavelength: 195nm.

[0072] And in step 3), after vortexing at room temperature for 30 minutes, centrifuge at 3500r / min for 8 minutes, filter the supernatant, extract repeatedly three times, combine the supernatant, pass the combined supernatant through a 0.22 μm filter membrane to obtain the sample to be tested liquid.

Embodiment 3

[0073] Embodiment 3: other content is all identical with embodiment 1, and difference is that the chromatographic condition in step 1) and step 4) is:

[0074] a) Chromatographic column: a BEH HILIC chromatographic column with a particle size of 2.5 μm and a specification of 4.6×150 mm;

[0075] b) Column temperature: 22°C;

[0076] c) mobile phase: water: acetonitrile (V: V) = 10:90 mixed solution, isocratic elution;

[0077] d) Mobile phase flow rate: 1.0mL / min;

[0078] e) Injection volume: 8 μL;

[0079] f) Detector type: fluorescence detector;

[0080] g) Detection wavelength: 195nm.

[0081] And in step 3), after vortexing at room temperature for 30 minutes, centrifuge at 4500r / min for 6 minutes, filter the supernatant, extract repeatedly three times, combine the supernatant, pass the combined supernatant through a 0.22 μm filter membrane to obtain the sample to be tested liquid.

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Abstract

The invention discloses a detection method for glycine betaine in aquatic products. Through a specific pretreatment method and specific high performance liquid chromatography conditions, the detection of glycine betaine in aquatic products is realized by using general high performance liquid chromatography equipment. The quantitative detection fills the gap in the detection method of glycine betaine in aquatic products, and compared with the previous method for detecting glycine betaine by colorimetry, the detection method of the present invention is more efficient and sensitive, and is suitable for popularization and application.

Description

technical field [0001] The invention relates to the field of detection of glycine betaine, in particular to a detection method of glycine betaine in aquatic products. Background technique [0002] Glycine betaine (glycine betaine) is one of the betaine compounds, the molecular formula is (CH 3 ) 3 NCH 2 COO. Glycine betaine is abundant in the muscles of aquatic products such as shrimp, crab, and shellfish. It is one of the main taste substances of these aquatic products and is also the main component that provides sweetness. Studies have shown that the content of glycine betaine plays a significant role in the taste differences of related aquatic products. [0003] At present, most of the detection methods for the content of glycine betaine use colorimetry. Although this method is simple to operate, it suffers from serious interference, low sensitivity and poor detection effect. In addition, aquatic products (including crabs, shrimps, shellfish, etc.) are not like goji ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 邢家溧丁源郑睿行张书芬承海周鑫达应璐毛玲燕
Owner 宁波市产品食品质量检验研究院(宁波市纤维检验所)