A kind of pseudorabies live vaccine diluent and its preparation method and application

A pseudorabies and diluent technology, applied in the field of pseudorabies live vaccine diluent and its preparation, can solve the problems of affecting the immune effect of nasal drops, insufficient vaccine virus quantity, and short antibody maintenance time, so as to prevent latent infection and improve drip immunity. Nasal immune effect, low-cost effect

Active Publication Date: 2022-03-04
云南生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Intranasal immunization of newborn piglets mainly plays an early occupying effect to prevent the invasion of wild viruses. Although it will not be interfered by maternal antibodies, the residence time of PBS buffer or saline as a diluent in the nasal cavity and respiratory tract is too short, and the occupying place Insufficient amount of vaccine virus also affects the effect of nasal drop immunity
In order to solve the above-mentioned problems such as the small number of vaccine viruses occupying the space and the interference of immunosuppressive diseases, low antibody titer, long production time, and short antibody maintenance time after immunization, this patented technology is proposed

Method used

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  • A kind of pseudorabies live vaccine diluent and its preparation method and application
  • A kind of pseudorabies live vaccine diluent and its preparation method and application
  • A kind of pseudorabies live vaccine diluent and its preparation method and application

Examples

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Embodiment 1

[0035] A method for preparing a live vaccine dilution of pseudorabies, comprising the steps of: taking microcrystalline cellulose and adding it into a reaction vessel with water for injection, stirring for 90 minutes at a temperature of 100° C. and a rotating speed of 200 rpm, and standing for 15 minutes. minutes, then add astragalus polysaccharide, stir and mix for 15 minutes, and sterilize at 115°C for 30 minutes to obtain a dilution of live pseudorabies vaccine. Wherein, every 1000 milliliters of said diluent contains 10 grams of microcrystalline cellulose and 15 grams of astragalus polysaccharide.

Embodiment 2

[0037] A method for preparing a live vaccine dilution of pseudorabies, comprising the steps of: taking microcrystalline cellulose and adding it into a reaction container with water for injection, stirring for 30 minutes at a temperature of 90°C and a rotation speed of 100rpm, and standing for 5 minutes minutes, then add astragalus polysaccharide, stir and mix for 5 minutes, and sterilize at 115°C for 30 minutes to obtain a dilution of live pseudorabies vaccine. Wherein, every 1000 milliliters of said diluent contains 1 gram of microcrystalline cellulose and 5 grams of astragalus polysaccharide.

Embodiment 3

[0039] A method for preparing a live vaccine dilution of pseudorabies, comprising the steps of: taking microcrystalline cellulose and adding it to a reaction vessel with water for injection, stirring for 45 minutes at a temperature of 100° C. and a rotating speed of 150 rpm, and standing for 12 minutes. minutes, then add astragalus polysaccharide, stir and mix for 8 minutes, and sterilize at 115°C for 30 minutes to obtain a dilution of live pseudorabies vaccine. Wherein, every 1000 milliliters of said diluent contains 3 grams of microcrystalline cellulose and 8 grams of astragalus polysaccharide.

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Abstract

The invention discloses a diluent of live pseudorabies vaccine, which contains microcrystalline cellulose and astragalus polysaccharide; and also discloses a method for preparing the diluent, which includes the following steps: taking microcrystalline cellulose and adding it to a reaction container filled with water for injection , stir, control the temperature, let stand, then add astragalus polysaccharide, stir and mix, sterilize, and obtain. The advantages of the present invention are: it can improve the effect of nasal drop immunity of pseudorabies vaccine, enhance the place-occupancy effect of pseudorabies virus in the cranial nerve and lymphatic system, intramuscular injection of immunization can produce antibodies in advance, prolong the maintenance time of high-level antibodies, and then improve Pig immunity. Effective prevention of latent infection of pseudorabies wild virus provides a guarantee for the prevention and eradication of pseudorabies. The preparation method of the diluent of the present invention has the characteristics of easy production, low cost, convenient use and the like.

Description

technical field [0001] The invention belongs to the technical field of vaccine processing, in particular to a diluent of pseudorabies live vaccine and its preparation method and application. Background technique [0002] my country is the largest breeding country in the world, with about 1 billion (heads) of pigs, cattle, and sheep slaughtered annually. In recent years, due to the ravages of animal infectious diseases, a large number of livestock and poultry deaths have been caused, which has seriously endangered the healthy development of the breeding industry. After the level of large-scale breeding is improved, the epidemic disease is more likely to spread, and the task of animal epidemic prevention and control is very arduous. However, in recent years, immunosuppressive diseases such as porcine ring disease and blue ear disease have been common in farmers all over the country, which has exacerbated the difficulty of preventing and controlling animal diseases. The phenom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/245A61P31/22A61K9/08A61K47/38A61K47/69A61K31/715
CPCA61K39/12A61K47/6949A61P31/22A61K9/0019A61K9/08A61K31/715C12N2710/16734A61K2039/543A61K2039/575A61K2039/552A61K2300/00
Inventor 肖华春李文春皇培刚章志宏王成业吴艳琴曹灿花马巧燕李斌赵志宏陈云才
Owner 云南生物制药有限公司
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