Method for preparing bovine skin gelatin by enzyme treatment

An enzyme treatment, cowhide technology, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of low gelatin yield, etc., and achieve the effect of cleaning and environmental protection in the production process.

Inactive Publication Date: 2019-02-19
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the final gelatin yield is lower (16.5%)

Method used

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  • Method for preparing bovine skin gelatin by enzyme treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The method for preparing cowhide gelatin by enzyme treatment comprises the following process steps:

[0055] A. Raw material pretreatment

[0056] Take the cowhide corner waste, cut into strips, add water to wash and remove impurities;

[0057] B. Enzyme hydrolysis reaction

[0058] Add distilled water to the cleaned leather prepared in step A, and bathe in water for 2 hours at a temperature of 50°C; add a compound enzyme composed of alkaline protease and alkaline lipase to the treated leather, wherein alkaline protease The amount of enzyme added is 500U per gram of raw hide, the amount of alkaline lipase added is 10U per gram of raw hide, and the enzymolysis reaction is carried out at pH = 8-10 and the temperature is 35 ° C for 10 hours;

[0059] C. Enzyme inactivation

[0060] After the enzymolysis reaction, discharge the enzymolysis solution, add water and stir for 5 minutes, remove the cleaning water, add distilled water again, and bathe in water for 10 minutes a...

Embodiment 2

[0068] The difference between this embodiment and embodiment 1 is:

[0069] B. Enzyme hydrolysis reaction

[0070] Add distilled water to the cleaned leather prepared in step A, and bathe in water for 10 minutes at a temperature of 80°C; add a compound enzyme consisting of alkaline protease and alkaline lipase to the treated leather, wherein alkaline protease The amount of enzyme added is 2000U per gram of raw hide, the amount of alkaline lipase added is 200U per gram of raw hide, and the enzymolysis reaction is carried out at pH = 8-10 and the temperature is 45°C for 6 hours;

[0071] C. Enzyme inactivation

[0072] After the enzymatic hydrolysis reaction, discharge the enzymatic hydrolysis solution, add water to stir and wash for 15 minutes, remove the washing water, add distilled water again, and bathe in water for 3 minutes at a temperature of 90°C;

[0073] D. Extraction

[0074] Place the material prepared in step C in a water bath at 60°C for 8 hours;

[0075] All t...

Embodiment 3

[0077] The difference between this embodiment and embodiment 1 is:

[0078] B. Enzyme hydrolysis reaction

[0079] Add distilled water to the cleaned leather prepared in step A, and bathe in water for 1 hour at a temperature of 70°C; add a compound enzyme consisting of alkaline protease and alkaline lipase to the treated leather, wherein alkaline protease The amount of enzyme added is 1500U per gram of raw hide, the amount of alkaline lipase added is 100U per gram of raw hide, and the enzymolysis reaction is carried out at pH = 8-10 and the temperature is 40°C for 8 hours;

[0080] C. Enzyme inactivation

[0081] After the enzymolysis reaction, discharge the enzymolysis solution, add water and stir to wash for 10 minutes, remove the cleaning water, add distilled water again, and bathe in water at 80°C for 7 minutes;

[0082] D. Extraction

[0083] The material prepared in step C was placed in a 70°C water bath for 6 hours;

[0084] All the other contents are the same as in...

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PUM

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Abstract

The invention relates to the preparation of gelatin, in particular to a method for preparing bovine skin gelatin by enzyme treatment, which comprises the process steps of raw material pretreatment, enzymolysis reaction, enzyme passivation, glue extraction, separation, concentration, drying and the like. The method solves the technical problems of low gelatin conversion rate, high post-treatment cost caused by pollution and the like in the prior art. The method has the advantages of high gelatin conversion rate, clean and environment-friendly approach, and that the indexes of the prepared gelatin meet the requirements of national standards.

Description

technical field [0001] The invention relates to the preparation of gelatin, in particular to a method for preparing cowhide gelatin by enzyme treatment. Background technique [0002] Gelatin has no fixed structure, and its molecular weight is between tens of thousands to hundreds of thousands. Gelatin is white or light yellow, mostly translucent flakes or powders. It is usually produced by the degradation of collagen in connective tissues such as bones, skin, tendons or membranes of pigs, cattle and other animals. Gelatin absorbs water and swells in cold water; it is soluble in hot water and coagulates into a gel after cooling; it is insoluble in organic solvents such as ethanol and chloroform, but soluble in acetic acid and glycerin. It can be stored for a long time in a dry state, and it is easy to deteriorate due to the action of bacteria after being damp. [0003] Gelatin has many advantages such as a wide range of raw materials, environmental protection and degradabi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/14
CPCC07K14/78C12P21/06
Inventor 高沛汝郑翔秦梦刘春卯秦艳梅马清河
Owner 河北省微生物研究所有限公司
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