Arbuscular mycorrhizal fungus spore enrichment and screening method and apparatus

Abstract

The invention relates to an arbuscular mycorrhizal fungus spore enrichment and screening apparatus, characterized in that filter cloth pieces are arranged in a tank so that a main culture vessel, a main enrichment vessel, an auxiliary enrichment vessel and an auxiliary culture vessel are formed in the tank, and the bottom of each of the main culture vessel and the auxiliary culture vessel is provided with drain holes. The main culture vessel is filled with a culture medium; an aseptic host plant is planted in the culture medium; the main enrichment vessel and the auxiliary culture vessel are filled with an aseptic medium, and host plants are planted in the main enrichment vessel and the auxiliary culture vessel; the auxiliary enrichment vessel is filled with aseptic river sand, and a nutrient liquid with a stress factor is applied; the culture medium is collected, and massive arbuscular mycorrhizal fungus spores with anti-stress characteristic are attained by means of a wet screen tilting process. The materials required herein and the culture mode are attainable in common labs; compared with the method of breeding arbuscular mycorrhizal fungus spores with hairy roots, the apparatusand method herein have no consideration on the spore pollution issue and are simple to perform and use; the apparatus is reusable and highly feasible.

Description

technical field [0001] The invention relates to a screening method and device, in particular to an arbuscular mycorrhizal fungus spore enrichment screening method and device, belonging to the field of microorganisms. Background technique [0002] Arbuscular mycorrhizal fungi are a kind of plant root symbiotic fungi that widely exist in soil and can form a symbiotic relationship with the roots of most higher plants. Arbuscular mycorrhizal fungal spores are reproductive organs of arbuscular mycorrhizal fungi. In order to amplify the spores of arbuscular mycorrhizal fungi, the most effective technique is to use artificial pure culture to amplify the spores of arbuscular mycorrhizal fungi. About 250 species of arbuscular mycorrhizal fungal spores have been discovered so far, belonging to the Glomus phylum, and traces of arbuscular mycorrhizal fungal spores can be seen in most adverse environments. In order to explore the characteristics of arbuscular mycorrhizal fungal spores ...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to disclose a method and device for enriching and screening arbuscular mycorrhizal fungal spores in order to overcome the defects of low efficiency and low content of arbuscular mycorrhizal fungal spores existing in the current arbuscular mycorrhizal fungal spore screening method

Images