Beet leaf paraffin-embedded sectioning method

Abstract

The invention belongs to the technical field of plant sectioning and discloses a beet leaf paraffin-embedded sectioning method, comprising: taking and immobilizing a material, to be specific, cuttingthe material, adding in a wide-mouth bottle, and immobilizing for 24 h; dewatering and transparentizing, to be specific, dewatering for 2 h, and transparentizing for 1.5 h; staining with safranin andfast green; refining with paraffin, to be specific, performing paraffin immersion with a 75 DEG C thermostatic box; using a semiautomatic embedding machine to perform embedding, placing the material in an embedding box, setting the material in position, and placing the embedding box on ice cubes made with an ice breaker for quick cooling; trimming, sectioning, sticking sections, and removing paraffin; sealing the sections, to be specific, sealing the sections in wet form, and drying by natural air. The inner tissue structure of beet leaf is made clear herein; the key steps, such as material taking and immobilizing, dewatering and transparentizing, paraffin melting, paraffin immersion, paraffin embedding, sectioning, section sticking, staining, and section making, are optimized; time is saved, slice making efficiency is improved, and success rate is increased.

Description

technical field [0001] The invention belongs to the technical field of plant tableting, and in particular relates to a method for making sugar beet leaf paraffin-embedded tablets. Background technique [0002] At present, the existing technology commonly used in the industry is this: the main product of sugar beets is sugar. Sugar is an indispensable nutrient for people's life, and it is also an important raw material for food industry, beverage industry and pharmaceutical industry. In addition to the production of sucrose, sugar beet and its by-products also have broad prospects for development and utilization. Beet leaf is the main growth and development organ of sugar beet, which mainly plays a role in photosynthesis. The petiole tissue section shows that the epidermis structure is tight, the petiole vascular column structure is clear and complete, the xylem, cambium, and phloem are clearly distinguishable, and the vessels and tracheids are dyed red , for mature lignifi...

AI Technical Summary

Problems solved by technology

[0005] (1) In the process of making paraffin slices in the prior art, there are often slices that are not banded, bent, cracked, shrunk, non-stick, falling off, and uneven in thickness

[0006] (2) In the prior art, different methods are adopted for different crops, different organs of the same crop, and different developmental stages of the same organ, which brings huge workload and reduces work efficiency

Images