The invention belongs to the technical field of
plant sectioning and discloses a beet leaf paraffin-embedded sectioning method, comprising: taking and immobilizing a material, to be specific, cuttingthe material, adding in a wide-mouth
bottle, and immobilizing for 24 h;
dewatering and transparentizing, to be specific,
dewatering for 2 h, and transparentizing for 1.5 h;
staining with safranin andfast green; refining with paraffin, to be specific, performing paraffin immersion with a 75 DEG C thermostatic box; using a semiautomatic embedding
machine to perform embedding, placing the material in an embedding box, setting the material in position, and placing the embedding box on ice cubes made with an ice breaker for quick cooling; trimming, sectioning, sticking sections, and removing paraffin; sealing the sections, to be specific, sealing the sections in wet form, and
drying by natural air. The inner tissue structure of beet leaf is made clear herein; the key steps, such as material taking and immobilizing,
dewatering and transparentizing, paraffin melting, paraffin immersion,
paraffin embedding, sectioning, section sticking,
staining, and section making, are optimized; time is saved, slice making efficiency is improved, and success rate is increased.