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Method for detecting volatile components of turtle oil

A technology of volatile components and detection methods, applied in the field of detection of volatile components of soft-shelled turtle oil, to achieve the effects of avoiding detection accuracy, high sensitivity, and shortening extraction time

Pending Publication Date: 2019-02-22
杭州邦沃森生物科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there are still some problems in HS-SPME, such as extraction time, control of extraction temperature, service life of extraction head, etc.

Method used

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  • Method for detecting volatile components of turtle oil
  • Method for detecting volatile components of turtle oil
  • Method for detecting volatile components of turtle oil

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but not as a basis for limiting the present invention.

[0018] Example. A method for detecting the volatile components of soft-shelled turtle oil, comprising the following steps:

[0019] ①Laser irradiation adsorption: Use a diode laser to irradiate the soft-shelled turtle oil sample. While the laser is irradiating the soft-shelled turtle oil, set an aged extraction head above the soft-shelled turtle oil, and use the extraction head to adsorb the volatilized substances;

[0020] ②Desorption: Insert the extraction head into the GC inlet of the gas chromatography-mass spectrometer, and desorb at 250°C for 3 minutes;

[0021] ③GC-MS detection: use gas chromatography-mass spectrometry for detection;

[0022] ④Compound analysis: through the NIST 2.0 spectral library for automatic search and confirmation analysis, and only when the positive and negative matc...

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Abstract

The invention discloses a method for detecting volatile components of turtle oil. According to the method, turtle oil samples are adsorbed and extracted in a laser radiation assisting mode, and detection is performed in a GC-MS detection method. The method for detecting the volatile components of the turtle oil is efficient, simple, convenient, low in cost, precise and comprehensive.

Description

technical field [0001] The invention relates to a method for detecting volatile components of soft-shelled turtle oil, belonging to the technical field of food detection. Background technique [0002] Soft-shelled turtle oil is rich in n-3 polyunsaturated fatty acids (n-3PUFA), mainly EPA and DHA, which have physiologically active functions such as anti-platelet aggregation, lowering blood lipids, and delaying thrombosis. It is a high-quality raw material for the development of advanced health food. However, crude soft-shelled turtle oil contains a large number of impurities, including free fatty acids (FFA), phospholipids, steroids, fat-soluble pigments, proteins, etc., especially the fishy smell substances produced by oxidative degradation of PUFA and rancidity of non-fat components in soft-shelled turtle oil, which have Certain toxic effects, and the presence of bad odor components in oils can easily reduce their physiologically active functions and sensory properties, th...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/12
CPCG01N30/02G01N30/06G01N30/12G01N2030/062G01N2030/128
Inventor 宋恭帅
Owner 杭州邦沃森生物科技有限公司
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