A filtration system that prevents cell rupture

A filtration system and cell technology, applied in the field of filtration systems to prevent cell rupture, can solve the problem of not considering whether cells rupture, etc., and achieve the effects of easy automatic control, convenient operation, and prevention of contamination of samples

Active Publication Date: 2021-10-08
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The filtration systems in the prior art do not take into account whether the filtered cells are ruptured, therefore, it is of great significance to design a filtration system that can prevent cell rupture

Method used

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  • A filtration system that prevents cell rupture
  • A filtration system that prevents cell rupture
  • A filtration system that prevents cell rupture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A filtration system that prevents cell rupture, see figure 1 , including filter 1, sample injection tube 2, liquid outlet tube 3, bubble detector 4, valve 5 and control part 6, details are as follows:

[0049] The sampling tube 2 is preferably made of a hose, such as polyvinyl chloride, and the sampling tube 2 communicates with the filter 1 to provide an inflow channel for the sample to enter the filter.

[0050] The liquid outlet pipe 3 communicates with the filter 1 and is used to provide an outflow channel for the filtered filtrate.

[0051] The bubble monitor 4 is arranged on the sampling tube 2, and is used to monitor whether there are bubbles in the sampling tube 2. The bubble monitor 4 is preferably an ultrasonic bubble monitor, such as Beijing Dongfang Jinrong Ultrasonic Electric Co., Ltd. Production of bubble monitors.

[0052] The valve 5 (a solenoid valve is used here) is arranged on the liquid outlet pipe 3, and is used to control whether the filtered filt...

Embodiment 2

[0059] The difference between this embodiment and embodiment 1 is that the filter structure is different, see image 3 ,specifically is:

[0060] 1. At least one of the bottom and the top of the filter 1 is provided with a good light-transmitting observation window; preferably, the bottom and the top of the filter 1 are provided with a good light-transmitting observation window 1.5, and the two Said observation window is arranged correspondingly, light can enter from one observation window, after reaching and passing through said filter membrane, exit from another observation window; The minimum distance from said filter membrane to the objective lens of the microscope through the observation window is greater than that of the microscope objective lens One focal length and infinitely close to one focal length; the observation window is an observation window with a flat surface, and the material with good light transmittance refers to a material with a light transmittance of mo...

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Abstract

The invention provides a filter system capable of preventing cell rupture, comprising a filter, a sample inlet tube, a liquid outlet tube, a bubble monitor, a valve and a control component, wherein a filter membrane is arranged in the filter; the sample inlet tube communicates with the filter; The liquid outlet pipe communicates with the filter; the bubble monitor is arranged on the sampling pipe; the valve is arranged on the liquid outlet pipe; both the air bubble monitor and the valve are connected with the control part. Applying the filtration system of the present invention, the effect is: the overall structure is simplified; through the combined design of the bubble monitor, the valve and the control part, the control part can control the switch of the valve according to the monitoring result of the bubble monitor, and automatically control the filtration process. The termination point ensures that the living cells trapped on the filter membrane are not directly in contact with the air, so that the trapped living cells remain active, which is convenient for the next step of research and analysis.

Description

technical field [0001] The invention relates to the technical field of filtration, in particular to a filtration system capable of preventing cell rupture. Background technique [0002] In the scientific research practice in the field of biomedicine, it is found that the fragmentation of the cell membrane is very easy to occur when the specific living cells are captured and filtered, resulting in the destruction of the entire cell, which has a great impact on further analysis after filtration: on the one hand, after the cell ruptures, the cells The outflow of internal fluid leads to cell death and the inability of cells to maintain activity, resulting in extremely low efficiency of filtering and separating living cells; This immunoassay and analysis has had significant adverse effects. [0003] Cell filtration systems in the prior art include: [0004] The utility model patent with the application number 201620130701.1 provides a filter system for filtering out rare circul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/12
CPCC12M47/04
Inventor 喻风雷王理胡琪康刘文亮文豪
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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