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A halo-tolerant salt field bacteria w-y11 and its application

A W-Y11, salt field bacteria technology, applied in bacteria, microorganisms, biochemical equipment and methods, etc., can solve problems such as crude oil degradation experiments that have not been carried out

Active Publication Date: 2022-04-05
北京润世能源技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The literature "Study on the Diversity of Microbial Community Diversity of Suaeda Pterosa Root System and Its Loading Performance to Oil-Breaking Bacteria" reported the strains belonging to Salinicola zeshunii after comparison, but no crude oil degradation experiment was carried out

Method used

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  • A halo-tolerant salt field bacteria w-y11 and its application
  • A halo-tolerant salt field bacteria w-y11 and its application
  • A halo-tolerant salt field bacteria w-y11 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1, Isolation, Identification and Preservation of W-Y11 Bacterial Strain

[0049] 1. Isolation of W-Y11 strain

[0050] In July 2017, oil-phase petroleum was obtained from the oil well production fluid of Shengli Oilfield in Karamay, Xinjiang, from which the inventor isolated a strain and named it strain W-Y11.

[0051] 2. Identification of W-Y11 strain

[0052] 1. Morphological identification of strain W-Y11

[0053] Morphological characteristics of the W-Y11 strain: flesh-colored round raised colonies were formed on LB plates containing 1% (mass fraction) sodium chloride.

[0054] 2. Physiological and biochemical identification of strain W-Y11

[0055] Physiological and biochemical characteristics of the W-Y11 strain: it can grow under the concentration of 0-20% sodium chloride.

[0056] 3. Molecular identification of strain W-Y11

[0057]The activated W-Y11 strain was inoculated in liquid LB medium containing 1% (mass fraction) sodium chloride (solvent is...

Embodiment 2

[0064] Embodiment 2, W-Y11 bacterial strain emulsifies heavy oil

[0065] The oil in this example was collected from Shengli Oilfield in Karama, Xinjiang in July 2017. The oil viscosity at 50°C is 28700mPa.s, which is extra heavy oil.

[0066] 1. Inoculate the activated W-Y11 into the liquid LB medium containing 1% (mass fraction) sodium chloride, culture at 30°C and 150rpm for 24 hours with shaking, and obtain the W-Y11 bacterial liquid (bacterial concentration ≥ 10 8 cfu / mL).

[0067] 2. Test group: Inoculate 5 parts by volume of bacterial liquid to 95 parts by volume of petroleum-inorganic salt medium (solvent is water, solute and its concentration are respectively 1g / L petroleum, 44g / L NaCl, 1g / L NH 4 h 2 PO 4 , 1g / L (NH 4 ) 2 SO 4 , 1g / L K 2 HPO 4 、3g / LKNO 3 , total mineralization degree 50000mg / L, pH 7-8), 30°C, 150rpm shaking culture for 7d.

[0068] Control group: 100 parts by volume of petroleum-inorganic salt medium were cultured with shaking at 30°C and 15...

Embodiment 3

[0071] Embodiment 3, the application of W-Y11 bacterial strain in microbial oil recovery technology

[0072] The oil in this example was collected from Shengli Oilfield in Karama, Xinjiang in July 2017. The oil viscosity at 50°C is 28700mPa.s, which is extra heavy oil.

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Abstract

The invention discloses a strain of salt-inhabiting salt field bacterium W-Y11 and its application. The taxonomy of the salt-tolerant salt field fungus W-Y11 of the present invention is named as the salt field fungus Salinicola sp., and the strain has been preserved in the General Microorganism Center of the China Microbiological Culture Collection Management Committee on November 13, 2018, and the preservation number is CGMCC No. .16741. It has been proved by experiments that the saline-tolerant halobacteria W-Y11 of the present invention can emulsify crude oil, and can not only be used for microbial oil recovery, improve oil recovery rate, but also be used for the control and repair of oil pollution, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of microbial oil recovery, in particular to a strain of saline-dwelling salt field bacterium W-Y11 and its application. Background technique [0002] Oil pollution is widely distributed, involving various industries such as oil extraction, oil refining, chemical industry, and machinery, as well as various polluting environments such as oil spills. Petroleum hydrocarbon components are complex and diverse, and are highly hydrophobic, making them difficult to be contacted, degraded, and utilized by ordinary microorganisms. Microbial oil recovery technology is to inject nutrients or microorganisms into the formation, and use the growth and metabolism activities of microorganisms in the oil reservoir to improve crude oil production and recovery. [0003] Traditional petroleum functional strains are mostly isolated from water and soil. Existing studies have shown that microorganisms are widely distributed in pet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K8/582A62D3/02C12R1/01A62D101/20
CPCA62D3/02C09K8/582A62D2101/20C12R2001/01C12N1/205
Inventor 池昌桥
Owner 北京润世能源技术有限公司
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