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Portable DNA extraction kit and extraction method

An extraction method and a portable technology, applied in the field of biomedicine, can solve the problems of inconvenient packaging and mailing, packaging damage, and affecting the reliability of test results, etc., to achieve the effect of improving collection efficiency and accuracy, improving efficiency, and improving further understanding and understanding

Inactive Publication Date: 2019-02-26
苏州市爱生生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the packaging of samples such as saliva or hair is inconvenient to mail, and it is easy to seriously affect the reliability of the test results due to problems such as packaging damage, leakage, and insufficient sample volume.

Method used

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  • Portable DNA extraction kit and extraction method
  • Portable DNA extraction kit and extraction method
  • Portable DNA extraction kit and extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Open the mobile App, enter the name and relevant information of the person to be sampled, and use the mobile App to scan the sample processing tube (such as figure 1 shown), the mobile app will automatically display the DNA extraction instructions (or instructions for use) for the customer.

[0027] Collect 5mL saliva samples, the specific steps are as follows: clean the mouth, stop eating, drinking, smoking and chewing gum 30 minutes before taking saliva samples. Collect 5 mL of saliva and add it to the sample processing tube (the sample processing tube contains 5 mL of sample processing solution sodium dodecylsulfonate (SDS) plus proteinase K), mix the saliva sample and sample processing solution thoroughly, and then statically Stand for 1 hour, and shake repeatedly by inversion during the standing process to fully mix the saliva and sample processing solution.

[0028] Add to the sample processing tube figure 2 The indicated DNA solid precipitation agent (sodium c...

Embodiment 2

[0034] Open the mobile App, enter the name and relevant information of the person to be sampled, use the mobile App to scan the QR code on the sample processing tube, and the mobile App will automatically display DNA extraction instructions (or instructions) for the customer.

[0035]Collect 5-10 hairs with follicles. Thoroughly mix the cut hair root with 10ml of the sample treatment solution, and then let it stand at room temperature for 1 hour. During the standing process, shake it upside down several times so that the hair follicle cells and the treatment solution can be fully mixed.

[0036] Add DNA solid precipitant sodium acetate (1 pack, 5 g) into the sample processing tube, and mix well.

[0037] Add 10ml DNA liquid precipitant ethanol to the sample processing tube and mix well.

[0038] Let the sample processing tube stand at room temperature for 15 minutes until a small transparent spherical DNA precipitate forms.

[0039] Use the DNA collection stick to slowly col...

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PUM

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Abstract

The invention provides a portable DNA extraction kit and a DNA extraction method. The efficiency of DNA sample collection is effectively improved and the corresponding inspection cost can be reduced.With the cooperative use of a mobile phone App, through instructions given by the mobile phone App, operational errors can be prevented and the collection efficiency and accuracy are improved. Sampleconfusion can be prevented by scanning two-dimensional codes on a sample processing tube and a DNA storage container of the kit by the mobile phone App. Especially, when multiple samples are processedsimultaneously, the accuracy of sample collection can be ensured.

Description

technical field [0001] The invention relates to a portable DNA extraction kit and a DNA extraction method, belonging to the field of biomedicine. Background technique [0002] In recent years, with the rapid development of DNA sequencing technology, the scope of genetic testing has been expanding. More and more people are now benefiting from genetic testing and the resulting precision medicine. To do genetic testing, the traditional method is to first go to the hospital to have blood collected by medical staff, and then the blood sample is sent to the laboratory to extract DNA for genetic testing. In recent years, direct-to-consumer genetic testing has gradually emerged. Traditional testing methods require sampling at testing institutions, which is less convenient. If a method that does not require sampling at a testing institution can be provided, it will be more convenient for DNA testing and easy to promote and use. For example, the subject can collect samples such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10G06K17/00
CPCC12N15/1003G06K17/0025C12Q2521/537
Inventor 杨亚平
Owner 苏州市爱生生物技术有限公司