Extraction process for active ingredients in leontopodium alpinum plant cell and application thereof

A technology of edelweiss alpine and plant cells, applied in the field of plant active ingredient extraction, can solve the problems of difficulty in extraction and loss of caffeoylquinic acids and the like

Pending Publication Date: 2019-03-01
TIANJIN ACELBIO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of large-scale loss of flavonoids, chlorogenic acid and caffeoylquinic acid substances caused by frozen storage of alpine edelweiss plant cell cultures in the prior art, and the problem that it is difficult to extract and obtain

Method used

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  • Extraction process for active ingredients in leontopodium alpinum plant cell and application thereof
  • Extraction process for active ingredients in leontopodium alpinum plant cell and application thereof
  • Extraction process for active ingredients in leontopodium alpinum plant cell and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The impact of embodiment 1 frozen storage time on the active ingredient content of alpine edelweiss plant cell culture

[0040] Harvest alpine edelweiss plant cell cultures, part of which were extracted from fresh cells immediately, and part of which were frozen at -20°C for 2-10 days for rethawing and extraction.

[0041] The method for extracting active ingredients from cell culture is as follows:

[0042] Add purified water to the fresh cells at a ratio of feed solution 1:5 (mass of fresh cells: volume of liquid added), and extract at 100°C for 1.0 h; the extraction method for cryopreserved cells is the same as above. Centrifuge the extracted extract at 6500rpm for 10min, retain the supernatant, and measure the total flavonoids, chlorogenic acid, 1,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid in the supernatant content. The test methods and test results from three different batches of cells are as follows:

[0043] The quantitative method of total flavono...

Embodiment 2

[0059] The extraction of embodiment 2 alpine edelweiss cell culture

[0060] Accurately weigh 100g of alpine edelweiss plant fresh cells, add 100mL of 80% ethanol at a material-to-liquid ratio of 1:1 (m / v), mix evenly, and perform ultrasonication for 20min in an ultrasonic device at a temperature of 30°C. Add 200mL of purified water twice the cell mass to the alpine edelweiss plant cell solution pretreated by ultrasonic wall breaking, and extract at a high temperature of 100°C for 0.5h, then centrifuge at 6500rpm for 10min, and carry out the supernatant Concentrate by rotation to a volume of 100 mL to obtain the cell culture extract of edelweiss alpine.

[0061] According to the total flavonoids quantitative method in Example 1, determine the total flavonoids content in the extract, standard curve, sample determination and calculation results are shown in Table 2:

[0062] Table 2 Quantitative analysis of extracts from alpine edelweiss plant cell culture

[0063]

[0064]...

Embodiment 3

[0074] The influence of embodiment 3 different extraction processes on the extract composition of alpine edelweiss plant cell culture

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Abstract

The invention provides a novel extraction process for active ingredients in a leontopodium alpinum plant cell culture. The process adopts flavonoids, chlorogenic acid and caffeoylquinic acids as extraction indexes, integrates ultrasonic and high-temperature extraction processes, and furthest retains and extracts the total flavonoids and caffeoylquinic acids in the leontopodium alpinum plant cell culture. After verifying that the extract has antioxidant, anti-inflammatory and anti-aging activities, the extract is used for the production of a skin external preparation.

Description

technical field [0001] The present invention relates to the field of extracting plant active ingredients, in particular to a process for extracting active ingredients from alpine edelweiss plant cell cultures, and more specifically relates to total flavonoids, chlorogenic acid and caffeoylquinone in alpine edelweiss plant cell cultures Extraction of nic acid active substance and its use in skin external preparations. Background technique [0002] Alpine edelweiss (Leontopodium alpinum) belongs to the Compositae family, and is a plant of the genus Edelweiss, also known as Alpine Thingrass and Little White Flower. It mainly grows in alpine and subalpine places at an altitude of 1400-3500m. Because it is rich in biologically active substances such as edelweiss acid, flavonoids, caffeoylquinic acids, and phenols, it is often used in the treatment of urinary system diseases and product preparations for whitening, skin care, anti-oxidation, and immune enhancement. [0003] In rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61K36/41A61P39/06A61P29/00A61P17/18
CPCA61K9/0014A61K36/074A61K36/28A61K36/41A61K2236/331A61K2236/51A61K2236/53A61P17/18A61P29/00A61P39/06A61K2300/00
Inventor 宋静静张卫陈萌王鹏刘永彬郭仪冰谭光伟
Owner TIANJIN ACELBIO BIOTECH
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