Magnetic nano-composite with antioxidant function and preparation method of magnetic nano-composite

Abstract

The invention discloses a magnetic nano-composite with an antioxidant function and a preparation method of the magnetic nano-composite, and belongs to the technical field of medical products. Magneticnanoparticles and amphipathic hyperbranched macromolecules are combined with antioxidant organic small molecule drugs to achieve magnetic performances and the antioxidant function. According to the magnetic nano-composite, a supramolecular chemical assembly method is used for performing supramolecular chemical driven self-assembly on polyethyleneimine (PEI)-graft-retinoic acid (RA) modified superparamagnetic nanoparticles and rutin molecules to obtain the magnetic nano-particle composite with the particle size of about 10nm and with the excellent antioxidant function and magnetic resonance imaging capacity. Rutin drugs with poor water solubility can be assembled with inorganic magnetic nanoparticles, and functions of the rutin drugs and the inorganic magnetic nanoparticles can be sufficiently achieved.

Description

technical field

[0001] The invention belongs to the field of medical products, and in particular relates to a magnetic nanocomposite with anti-oxidation function and a preparation method thereof. Background technique

[0002] The cells, tissues or organs of the human body continuously undergo various redox reactions in various free radicals, and produce products of human metabolism such as reactive oxygen species (ROS) and reactive nitrogen species (RNS). The human body maintains the oxidative balance in the body through its own antioxidant system and various antioxidant enzymes, and generates oxidative stress during this process. Oxidative stress is considered to be one of the causes of various acute and chronic diseases such as cancer, Parkinson's, and cardiovascular diseases. Not only that, tumor patients have more oxidative stress in vivo and affect the progression of the disease. As a specific marker of oxidative damage in vivo, protein oxidation products mediated by ...

Examples

Embodiment 1

[0040](1) Synthesis of PEI-graft-RA complex: Activate 50 mg RA in 10 mL of anhydrous DMSO with 35 mg of DCC and 20 mg of HoSu, then add 10 mL of DMSO solution in which 1 g of PEI was dissolved, and activate under a protective atmosphere in the dark for 12 hours. The synthesized product was dialyzed and freeze-dried to obtain the PEI-graft-RA complex.

[0041] (2) Preparation of a suspension of superparamagnetic particles: take 15 mg of superparamagnetic particles, disperse them in 15 mL of water, and keep the concentration of the suspension at 1 mg / mL;

[0042] (3) Mix the suspension obtained in step (2) with 15 mg of commercial rutin, and react at room temperature for 24 hours to obtain a superparamagnetic nanoparticle complex with anti-oxidation function.

[0043] (4) After dialysis of the reaction mixture in step (3), centrifuge at 5000 rpm for 20 min to obtain the product and redisperse it in PBS to form a stable dispersion system to obtain the desired contrast agent.

[...

Embodiment 2

[0047] (1) Synthesis of PEI-graft-RA complex: Activate 100 mg RA in 10 mL of anhydrous DMSO with 70 mg of DCC and 40 mg of HoSu, then add 10 mL of DMSO solution in which 1 g of PEI was dissolved, and activate under a protective atmosphere in the dark for 12 hours. The synthesized product was dialyzed and freeze-dried to obtain the PEI-graft-RA complex.

[0048] (2) Preparation of a suspension of superparamagnetic particles: take 15 mg of superparamagnetic particles, disperse them in 15 mL of water, and keep the concentration of the suspension at 1 mg / mL;

[0049] (3) Mix the suspension obtained in step (2) with 15 mg of commercial rutin, and react at room temperature for 24 hours to obtain a superparamagnetic nanoparticle complex with anti-oxidation function.

[0050] (4) After dialysis of the reaction mixture in step (3), centrifuge at 5000 rpm for 20 min to obtain the product and redisperse it in PBS to form a stable dispersion system to obtain the desired contrast agent.

...

Embodiment 3

[0053] (1) Synthesis of PEI-graft-RA complex: Activate 75mg RA in 10mL of anhydrous DMSO by 52.5mg of DCC and 30mg of HoSu, then add 10mL of DMSO solution with 1g of PEI, and keep the activation reaction under a protective atmosphere for 12h in the dark , the synthesized product was dialyzed and freeze-dried to obtain the PEI-graft-RA complex.

[0054] (2) Preparation of a suspension of superparamagnetic particles: take 15 mg of superparamagnetic particles, disperse them in 15 mL of water, and keep the concentration of the suspension at 1 mg / mL;

[0055] (3) Mix the suspension obtained in step (2) with 15 mg of commercial rutin, and react at room temperature for 24 hours to obtain a superparamagnetic nanoparticle complex with anti-oxidation function.

[0056] (4) After dialysis of the reaction mixture in step (3), centrifuge at 5000 rpm for 20 min to obtain the product and redisperse it in PBS to form a stable dispersion system to obtain the desired contrast agent.

[0057] W...

technical field

[0001] The invention belongs to the field of medical products, and in particular relates to a magnetic nanocomposite with anti-oxidation function and a preparation method thereof. Background technique

[0002] The cells, tissues or organs of the human body continuously undergo various redox reactions in various free radicals, and produce products of human metabolism such as reactive oxygen species (ROS) and reactive nitrogen species (RNS). The human body maintains the oxidative balance in the body through its own antioxidant system and various antioxidant enzymes, and generates oxidative stress during this process. Oxidative stress is considered to be one of the causes of various acute and chronic diseases such as cancer, Parkinson's, and cardiovascular diseases. Not only that, tumor patients have more oxidative stress in vivo and affect the progression of the disease. As a specific marker of oxidative damage in vivo, protein oxidation products mediated by ...

Examples

Embodiment 1

[0040](1) Synthesis of PEI-graft-RA complex: Activate 50 mg RA in 10 mL of anhydrous DMSO with 35 mg of DCC and 20 mg of HoSu, then add 10 mL of DMSO solution in which 1 g of PEI was dissolved, and activate under a protective atmosphere in the dark for 12 hours. The synthesized product was dialyzed and freeze-dried to obtain the PEI-graft-RA complex.

[0041] (2) Preparation of a suspension of superparamagnetic particles: take 15 mg of superparamagnetic particles, disperse them in 15 mL of water, and keep the concentration of the suspension at 1 mg / mL;

[0042] (3) Mix the suspension obtained in step (2) with 15 mg of commercial rutin, and react at room temperature for 24 hours to obtain a superparamagnetic nanoparticle complex with anti-oxidation function.

[0043] (4) After dialysis of the reaction mixture in step (3), centrifuge at 5000 rpm for 20 min to obtain the product and redisperse it in PBS to form a stable dispersion system to obtain the desired contrast agent.

[...

Embodiment 2

[0047] (1) Synthesis of PEI-graft-RA complex: Activate 100 mg RA in 10 mL of anhydrous DMSO with 70 mg of DCC and 40 mg of HoSu, then add 10 mL of DMSO solution in which 1 g of PEI was dissolved, and activate under a protective atmosphere in the dark for 12 hours. The synthesized product was dialyzed and freeze-dried to obtain the PEI-graft-RA complex.

[0048] (2) Preparation of a suspension of superparamagnetic particles: take 15 mg of superparamagnetic particles, disperse them in 15 mL of water, and keep the concentration of the suspension at 1 mg / mL;

[0049] (3) Mix the suspension obtained in step (2) with 15 mg of commercial rutin, and react at room temperature for 24 hours to obtain a superparamagnetic nanoparticle complex with anti-oxidation function.

[0050] (4) After dialysis of the reaction mixture in step (3), centrifuge at 5000 rpm for 20 min to obtain the product and redisperse it in PBS to form a stable dispersion system to obtain the desired contrast agent.

...

Embodiment 3

[0053] (1) Synthesis of PEI-graft-RA complex: Activate 75mg RA in 10mL of anhydrous DMSO by 52.5mg of DCC and 30mg of HoSu, then add 10mL of DMSO solution with 1g of PEI, and keep the activation reaction under a protective atmosphere for 12h in the dark , the synthesized product was dialyzed and freeze-dried to obtain the PEI-graft-RA complex.

[0054] (2) Preparation of a suspension of superparamagnetic particles: take 15 mg of superparamagnetic particles, disperse them in 15 mL of water, and keep the concentration of the suspension at 1 mg / mL;

[0055] (3) Mix the suspension obtained in step (2) with 15 mg of commercial rutin, and react at room temperature for 24 hours to obtain a superparamagnetic nanoparticle complex with anti-oxidation function.

[0056] (4) After dialysis of the reaction mixture in step (3), centrifuge at 5000 rpm for 20 min to obtain the product and redisperse it in PBS to form a stable dispersion system to obtain the desired contrast agent.

[0057] W...

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