Fruit and vegetable composite biological preservative and preparation method thereof
A biological preservative, fruit and vegetable technology, applied in the direction of fruit and vegetable preservation, food preservation, food science, etc., can solve the problems of prolonging the storage time of fruits and vegetables, weakening the respiration intensity of fruits and vegetables, reducing the water dispersion of fruits and vegetables, etc., to achieve the purpose of inhibiting the production of mycotoxins, The effect of maintaining hardness and reducing water evaporation
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[0038] The preparation method of the fruit and vegetable compound biological preservative in the present embodiment comprises the following steps:
[0039] S1. Culture of microbial fermentation broth: a. Activation: Inoculate Candida intertype and Cryptococcus laurentis with a mass ratio of 1:1 on NYDA plate medium, and cultivate at 30°C for 16-20 hours , repeated subculture twice; b. cultivation: inoculate the activated yeast in NYDB liquid medium, culture at 150r / min, 30°C for 22 hours; c. centrifugation: centrifuge the yeast culture solution at 7000r / min for 10min, discard the upper Clear liquid, rinsed with sterile water for 3 times, counted live bacteria on the hemocytometer, and adjusted the concentration to 1×10 6 -1×10 9 Individual / mL bacterial suspension, to obtain;
[0040] S2. Pulverize 10 g of citrus peels, soak them in 300 mL of ethanol solution with a concentration of 75%, then microwave extraction for 0.5 h at a power of 700W and a temperature of 80°C, then ad...
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