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A strain of Citrobacter zyd-1 and its application in the degradation of hexabromocyclododecane

A technology of hexabromocyclododecane and citric acid bacteria, which is applied to the restoration of bacteria and contaminated soil, and methods based on microorganisms, to achieve the effect of simple operation process and low requirements for management conditions

Active Publication Date: 2021-09-24
NANKAI UNIV
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Problems solved by technology

However, there are still very few studies on the microbial degradation of HBCD so far, and there is a lack of research on the biodegradation of different HBCD isomers alone and the degradation products and degradation pathways of different HBCD isomers.

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  • A strain of Citrobacter zyd-1 and its application in the degradation of hexabromocyclododecane
  • A strain of Citrobacter zyd-1 and its application in the degradation of hexabromocyclododecane
  • A strain of Citrobacter zyd-1 and its application in the degradation of hexabromocyclododecane

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Embodiment Construction

[0031] The Citrobacter sp.ZYD-1 provided by the present invention was preserved on November 2, 2018 in the General Microbiology Center of the China Microbiological Culture Collection Management Committee (No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Microbiological Research, Chinese Academy of Sciences Institute), preservation number CGMCC No.16678, proposed to be classified as Citrobacter sp.. The bacterial strain is a Gram-negative bacterium, white, and is a grayish-white opaque circular colony raised on the surface. It is rod-shaped under a scanning electron microscope, with a diameter of 0.5-0.7 μm ×1-2μm. The scanning electron microscope and flow cytometry pictures are attached figure 1 And attached figure 2 shown.

[0032] The screening method of Citrobacter ZYD-1 of the present invention, concrete steps are as follows:

[0033] Soil samples of e-waste were collected in May 2016 from the recycling and dismantling site of waste electronic information pr...

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Abstract

A strain of Citrobacter ZYD‑1 and its application in the degradation of hexabromocyclododecane, the strain is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.16788. The strain is a Gram-negative, white, off-white opaque round colony with a raised surface. Under the scanning electron microscope, it is rod-shaped, with a diameter of 0.5-0.7μm×1-2μm. The facultative anaerobic degrading bacterium ZYD-1 screened in the present invention can use HBCD as the sole carbon source, and has a relatively high degradation rate for HBCD. It was also found that during the anaerobic degradation process, there is a phenomenon of conversion of γ-HBCD to α-HBCD. The invention can be used for the restoration of HBCD polluted sites, especially for anaerobic environments such as sediments and bottom mud.

Description

technical field [0001] The invention belongs to the application technical field of microorganisms and the repairing of HBCD pollution. The invention relates to a screening method for a new bacterial strain Citrobacter with HBCD degrading performance, and a method for degrading HBCD and its application. Background technique [0002] As a commonly used brominated flame retardant, hexabromocyclododecane (HBCD) is the third largest brominated flame retardant after polybrominated diphenyl ether and bisphenol A. Its molecular formula is C 12 h 18 Br 6 , the bromine content reached 74.7%. There are three main isomers, namely α-HBCD, β-HBCD, and γ-HBCD. Industrial-grade HBCD is mainly γ-HBCD, and the isomers can interact with each other when the temperature is above 160°C. transform. Because of its high bromine content, good flame retardancy, low dosage, low cost, and little impact on material properties, it is an excellent additive flame retardant. Therefore, it has a large ma...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/36
CPCB09C1/10C02F3/34C02F2101/36C12N1/20C12N1/205C12R2001/01
Inventor 王莹莹张娅迪韩伟杨昭
Owner NANKAI UNIV
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