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Genetic engineering bacterium for PET plastic degrading

A technology of genetically engineered bacteria and plastics, applied in the field of genetically engineered bacteria that degrade PET plastics, can solve problems such as the inability to completely degrade PET plastics

Pending Publication Date: 2019-03-01
深伦生物科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a genetically engineered bacterium for degradation of PET plastics, aiming to solve the problem that the hydrolytic enzymes secreted by existing engineering bacteria cannot completely degrade PET plastics

Method used

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Embodiment Construction

[0021] The present invention provides a genetically engineered bacterium for degradation of PET plastics. In order to make the purpose, technical solution and effect of the present invention clearer and more definite, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] In the prior art, the construction of engineering bacteria that exclusively secretes PETase can only degrade PET plastic from a high polymer state to an oligomer or oligomer state, and it cannot degrade PET plastic into phthalic acid and ethylene glycol mono body, so it cannot achieve complete degradation of PET plastics.

[0023] Based on this, the present invention provides a genetically engineered bacterium for the degradation of PET plastics, wherein the genetically engineered bacterium includes gene fragments expressing PET hydrolase a...

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Abstract

The invention discloses a genetic engineering bacterium for PET plastic degrading. The genetic engineering bacterium is acquired by transferring a gene segment used for expressing PET hydrolase and MHET hydrolase into a host bacterium in the manner of double-plasmid double expression or single-plasmid double expression. The genetic engineering bacterium provided by the invention is capable of simultaneously expressing PET hydrolase and MHET hydrolase and secreting out of cells; the PET hydrolase is capable of degrading PET plastic to oligomer or low polymer state; the MHET hydrolase is capableof degrading PET plastic from oligomer or low polymer state to phthalic acid and glycol monomer, so as to realize full degradation of PET plastic. The invention provides an effective biodegradation method for full degradation of PET plastic.

Description

technical field [0001] The invention relates to the field of gene recombination, in particular to a genetically engineered bacterium for degrading PET plastics. Background technique [0002] In the prior art, most of the degradation methods of PET (polybutylene terephthalate) plastics are landfill, incineration, etc., and these processing methods are likely to cause serious pollution to the environment. Researchers have constructed engineering bacteria that exclusively secrete PETase (PET hydrolase), but PETase can only degrade PET plastic from a high polymer state to an oligomer or oligomer state, and it cannot degrade PET plastic to Phthalic acid and ethylene glycol monomers cannot completely degrade PET plastics. [0003] Therefore, prior art still needs to improve and develop. Contents of the invention [0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a genetically engineered bacterium for degrada...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/70C12R1/19
CPCC12N9/18C12N15/70
Inventor 方崇洲周光前李明苏庆宁韩倩莫翠萍周焱
Owner 深伦生物科技(深圳)有限公司
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