Single-target gene editing T0 generation tobacco plant screening method

Abstract

The invention discloses a single-target editing T0 generation tobacco plant screening method. The single-target editing T0 generation tobacco plant screening method comprises the steps of 1, adoptinga palm computer to collect sample information, and connecting the palm computer with a server terminal wirelessly; 2, managing tobacco leaf samples according to the result of wireless connection between the palm computer and the server terminal, screening tobacco plants which need tobacco leaf sample connection in real time, collecting, signing in, treating, storing, distributing, detecting and the tobacco leaf samples, storing and treating remained detected samples, feeding back detection results to a tobacco plant planting staff, and completing screening of the single-target gene editing T0generation tobacco plants; the T0 generation tobacco plants are gene editing regrowth tobacco plants. By adopting the screening method, the similar single-target gene editing T0 generation tobacco plants in large quantity can be screened efficiently and precisely.

Description

Technical field [0001] The invention relates to the field of computer software, in particular to a method for screening a large number of tobacco plants for high-efficiency and accurate single-target gene editing T0 generation in gene editing breeding. Background technique [0002] Gene editing breeding technology has been widely used in the genome modification of various plants, such as Arabidopsis, rice and tobacco. With the establishment of tobacco gene editing breeding plants and the rapid advancement of material creation, a large number of gene editing materials have been created. These editing materials have become the focus of attention in tobacco plant phenotype screening, but the chemical composition and physiology of tobacco leaves similar to tobacco plant phenotypes There are differences in biochemical indicators. Therefore, how to efficiently and accurately select tobacco plants from a large amount of edited materials has become an urgent problem to be solved. [0003...

AI Technical Summary

Problems solved by technology

[0003] Since the screening of tobacco strains involves multiple steps such as tobacco strain selection, tobacco leaf sample collection, processing and analysis feedback, including tobacco strain selection that requires tobacco leaf sample collection, tobacco strain sampling marking, special phenotype recording, tobacco leaf collection and bagging, and tobacco leaf freezing , freeze-drying of tobacco leaves, grinding of tobacco leaves, storage of tobacco leaf powder, testing and processing of residual samples, and finally re-screening of massive tobacco strains according to the feedback of the test results of tobacco leaves. Traditionally, manual use of a marker pen is used to copy the code of the tobacco plant to the sample bag or sample tube, and then gradually enter the code into the computer terminal. These operations require high personnel quality and are prone to various errors, which seriously affect the sample management process. Efficient and accurate screening of editing materials affecting tobacco leaves

At the same time, there is a large randomness in the sampling and pre-processing process. If there is no unified management, it will bring instability to the subsequent detection.

These have seriously affected the establishment of tobacco gene editing breeding factories and the rapid advancement of material creation