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Exosome preparation for maintaining and recovering DPC (Dermal Papilla Cell) stemness

A dermal papilla cell and system technology, applied in artificial cell constructs, animal cells, vertebrate cells, etc., can solve the problems of high cost, difficult to pass, and high immunogenicity of the body, so as to reduce the dosage, reverse the original stemness, The effect of reducing side effects

Active Publication Date: 2019-03-08
浙江卫未生物医药科技有限公司
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  • Description
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  • Application Information

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Problems solved by technology

However, it is difficult to apply T cells in the clinical hair loss treatment directly through cell transplantation, and there are problems such as high immunogenicity of the body; and it is necessary to restore high-passage DPC through conventional co-culture methods, such as transwell co-culture Dry, there is a problem of high cost

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  • Exosome preparation for maintaining and recovering DPC (Dermal Papilla Cell) stemness
  • Exosome preparation for maintaining and recovering DPC (Dermal Papilla Cell) stemness
  • Exosome preparation for maintaining and recovering DPC (Dermal Papilla Cell) stemness

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Embodiment Construction

[0036] The present invention will be further described in detail below in conjunction with the accompanying drawings, and specific implementation methods will be given.

[0037]The present invention is an exosome preparation for maintaining and restoring the stemness of dermal papilla cells. The exosome preparation can be used to prepare a culture medium. The exosome preparation is prepared and applied by the following method:

[0038] 1) Separation and acquisition of DPC, the unit hair follicle tissue in the posterior occipital area of ​​the patient is collected, and the improved two-step enzyme digestion method is used to separate and cultivate DPC in the laboratory, specifically: (1) A single hair follicle after surgery is placed in the hair follicle collection solution Store at 4°C, wash the hair follicle tissue with 1% double-antibody PBS solution for 2 to 3 times; (2) Use a 1ml syringe needle to separate the entire hair bulb tissue under a microscope, and remove the remai...

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Abstract

The invention discloses an exosome preparation for maintaining and recovering DPC (Dermal Papilla Cell) stemness. The preparation is prepared by the following methods: (1) separating to obtain DPCs; (2) separating mononuclear cells from peripheral blood; (3) screening CD4+CD25+Foxp3+Treg cells; (4) acquiring a CD4+CD25+Foxp3+Treg cell conditioned medium; (5) preparing a DPC inducing medium; (6) performing induced culture on the DPCs; (7) preparing the exosome preparation. According to the method, the problems of immunological rejection of the CD4+CD25+Foxp3+Treg cells in direct application (cell transplantation) and high co-culture cost in an alternative method (transwell) are avoided; high-generation DPC stemness can be recovered in DPC culture.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and in particular relates to an exosome preparation for maintaining and restoring the stemness of dermal papilla cells. Background technique [0002] Dermal papilla cells (DPCs) are critical for hair follicle growth, signaling the initiation of follicle development and hair cycle turnover. The current common hair loss in men and women is manifested as progressive hair follicle miniaturization including dermal papilla tissue, resulting in shortened hair growth cycle, disordered hair follicle cycle, shortened growth period, and thinner and shorter hair. [0003] At present, some researchers have isolated DPCs that play a key role in hair follicle regeneration, and found that under in vitro culture conditions, DPCs with lower algebra than the original ones grow aggregated, express alkaline phosphatase (Alkaline phosphatase, ALP) and α-smooth muscle protein (α-SMA) and other unique marker mole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/0783
CPCC12N5/0627C12N5/0636C12N2509/00
Inventor 袁博陈锦阳刘军权
Owner 浙江卫未生物医药科技有限公司