Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A ru(bpy) based 3 2+ The preparation method of the immunosensor of /zn-oxalate

A sensor and luminescence immunosensor technology, applied in the preparation of double-quenched sandwich type electrochemiluminescence immunosensor, and the application field of electrochemiluminescence sensor

Active Publication Date: 2021-07-06
UNIV OF JINAN
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently based on Ru(bpy) 3 2+ / Zn-oxalate MOFs to construct a dual-quenching immunosensor to detect Aβ has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A ru(bpy) based <sub>3</sub> <sup>2+</sup> The preparation method of the immunosensor of /zn-oxalate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Ru(bpy) 3 2+ / Zn-oxalated MOFs labeled β-amyloid primary antibody incubation solution, Au@NiFeMOFs labeled β-amyloid secondary antibody incubation solution

[0034] (1) Ru(bpy) 3 2+ / Preparation of Zn-oxalated MOFs

[0035] Add 10 mg terpyridine ruthenium chloride hexahydrate and 80 mg zinc nitrate to a 1:1 mixture of N, N-dimethylformamide and ultrapure water at the same time, and continue to add 200 μL, 0.75 mol L -1 Oxalic acid (, 2 mL, 3 mol L -1 Hydrochloric acid, ultrasonic at room temperature for 5 min, transfer the mixture to a round-bottomed flask, reflux at 60 °C for 12 h, centrifuge the suspension, wash three times with N, N-dimethylformamide and ultrapure water respectively to obtain an orange-red precipitate, Vacuum drying at 35°C yields Ru(bpy) 3 2+ / Zn-oxalated MOFs complex;

[0036] (2) Ru(bpy) 3 2+ / Preparation of Zn-oxalated MOFs labeled β-amyloid primary antibody incubation solution

[0037] Add 10 mmol EDC and 5 mmol NHS to 1 mL, 1 mg mL...

Embodiment 2

[0044] Ru(bpy) 3 2+ / Zn-oxalated MOFs labeled β-amyloid primary antibody incubation solution, Au@NiFeMOFs labeled β-amyloid secondary antibody incubation solution

[0045] (1) Ru(bpy) 3 2+ / Preparation of Zn-oxalated MOFs

[0046] Add 15 mg terpyridine ruthenium chloride hexahydrate and 100 mg zinc nitrate to a 2:1 mixture of N, N-dimethylformamide and ultrapure water at the same time, and continue to add 300 μL, 0.75 mol L -1 Oxalic acid, 3 mL, 3 mol L -1 Hydrochloric acid, ultrasonic at room temperature for 5 min, transfer the mixture to a round-bottomed flask, reflux at 60 °C for 24 h, centrifuge the suspension, wash three times with N, N-dimethylformamide and ultrapure water respectively to obtain an orange-red precipitate, Vacuum drying at 35°C yields Ru(bpy) 3 2+ / Zn-oxalated MOFs complex;

[0047] (2) Ru(bpy) 3 2+ / Preparation of Zn-oxalated MOFs labeled β-amyloid primary antibody incubation solution

[0048] Add 20 mmol EDC and 10 mmol NHS to 2 mL, 1 mg mL...

Embodiment 3

[0055] Ru(bpy) 3 2+ / Zn-oxalated MOFs labeled β-amyloid primary antibody incubation solution, Au@NiFeMOFs labeled β-amyloid secondary antibody incubation solution

[0056] (1) Ru(bpy) 3 2+ / Preparation of Zn-oxalated MOFs

[0057] Add 20 mg terpyridine ruthenium chloride hexahydrate and 120 mg zinc nitrate to a 3:1 mixture of N, N-dimethylformamide and ultrapure water at the same time, and continue to add 400 μL, 0.75 mol L -1 Oxalic acid, 4 mL, 3 mol L -1 Hydrochloric acid, ultrasonic at room temperature for 5 min, transfer the mixture to a round-bottomed flask, reflux at 60 °C for 36 h, centrifuge the suspension, wash three times with N, N-dimethylformamide and ultrapure water respectively to obtain an orange-red precipitate, Vacuum drying at 35°C yields Ru(bpy) 3 2+ / Zn-oxalated MOFs complex.

[0058] (2) Ru(bpy) 3 2+ / Preparation of Zn-oxalated MOFs labeled β-amyloid primary antibody incubation solution

[0059] Add 30 mmol EDC and 15 mmol NHS to 3 mL, 1 mg mL...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a new nanoporous material based on Ru(bpy) 3 2+ / Zn‑oxalate MOFs double quenching electrochemiluminescence immunosensor preparation method, belongs to the field of electrochemiluminescence sensors, the first nanocomposite material Ru(bpy) 3 2+ / Zn-oxalate MOFs as the source of the electrochemiluminescence signal, using another nanoporous material Au@NiFe MOFs as a double quencher to construct a sandwich-type immunosensor, according to the intensity of the electrochemiluminescence signal caused by different concentrations of β-amyloid Different, realize the detection of β-amyloid protein.

Description

technical field [0001] The present invention relates to a kind of based on Ru(bpy) 3 2+ / Zn-oxalate MOFs double quenching sandwich electrochemiluminescence immunosensor preparation method. Specifically related to the luminescent material Ru(bpy) 3 2+ Preparation of / Zn-oxalate MOFs and nanoporous Au@NiFeMOFs and their application in electrochemiluminescence sensors. Au@NiFeMOFs with high porosity, large specific surface area, and good electrical conductivity were used as dual quenchers to label antibodies to achieve dual quenching of chemiluminescent signals. The invention belongs to the technical field of electrochemiluminescence detection. Background technique [0002] Soluble oligomers of β-amyloid (Aβ) isolated from the cytoplasmic matrix of Alzheimer's disease (AD) brains have been shown to impair hippocampal extrinsic plasticity, reduce synapses, and induce tau hyperphosphorylation , can also cause symptoms such as neurodystrophy, activated microglial inflammatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N21/76G01N27/30
CPCG01N21/76G01N27/30G01N33/531
Inventor 赵冠辉曹伟王耀光李小建东雪房靖龙魏琴
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com