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Application of bcdmt2 protein and its coding gene in regulating pathogenicity and conidia production of Botrytis cinerea

A technology of conidia and coding genes, which is applied in the fields of plant pathology and microbial genetic engineering, can solve the problems of reduced drug efficacy and achieve broad application prospects

Active Publication Date: 2021-04-16
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The long-term use of chemical pesticides leads to the emergence of pathogenic bacteria resistance, which significantly reduces the efficacy of the pesticides

Method used

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  • Application of bcdmt2 protein and its coding gene in regulating pathogenicity and conidia production of Botrytis cinerea
  • Application of bcdmt2 protein and its coding gene in regulating pathogenicity and conidia production of Botrytis cinerea
  • Application of bcdmt2 protein and its coding gene in regulating pathogenicity and conidia production of Botrytis cinerea

Examples

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Effect test

Embodiment 1

[0048] Example 1, Construction and Phenotype Analysis of Bcdmt2 Knockout Mutants

[0049] 1. Construction of Bcdmt2 knockout mutant strain

[0050] Using the principle of homologous recombination of gene flanking sequences, the Bcdmt2 gene on the Botrytis cinerea genome was replaced with the hygromycin B resistance gene, so as to achieve the purpose of knocking out the Bcdmt2 target gene. The knockout strategy is as follows: figure 2 shown. Specific steps are as follows:

[0051] 1. Construction of knockout vector

[0052] Use the upstream and downstream sequences of the Bcdmt2 target gene as a template to design primers to amplify the upstream and downstream recombination arms (500-1000bp is appropriate), use the pLOB7 vector as a template to amplify a hygromycin-resistant fragment, and use fusion PCR to amplify the upstream and downstream recombination arms. The recombination arms are connected to both ends of the hygromycin-resistant fragment. Specific steps are as fol...

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Abstract

The invention discloses the application of the Bcdmt2 protein and its coding gene in regulating the pathogenicity of Botrytis cinerea and the production of conidia. The gene Bcdmt2 provided by the invention is derived from Botrytis cinerea, and plays an important role in vegetative growth, production of conidia and pathogenic process. The knockout experiment of Bcdmt2 in wild-type Botrytis cinerea found that the pathogenicity, germination rate of conidia and spore production of Botrytis cinerea were significantly reduced after knockout of Bcdmt2. It shows that Bcdmt2 is not only an important regulatory gene of Botrytis cinerea pathogenicity, but also a key gene for conidia production of Botrytis cinerea, which has broad application prospects in the control of plant pathogenic fungal diseases.

Description

technical field [0001] The invention relates to the fields of plant pathology and microbial genetic engineering, in particular to the application of Bcdmt2 protein and its coding gene from Botrytis cinerea in regulating the vegetative growth, conidia production and pathogenic process of Botrytis cinerea. Background technique [0002] Gray mold is a disease that causes great harm to agricultural production. It is an important disease of many important economic crops, such as grapes, strawberries, tomatoes, roses, etc., during pre-harvest production and post-harvest storage. Botrytis cinerea is the causative agent of Botrytis cinerea, which belongs to the subphylum Ascomycota and Botrytis cinerea. B.cinerea is a necrotrophic pathogenic fungus with a wide range of hosts, which can infect more than 200 kinds of plants. B. cinerea mainly in the form of sclerotium in the soil or overwintering or oversumming on the diseased residue as mycelia and conidia. When the conditions are s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/37C12N1/15C12N15/90C12N15/11A01N57/16A01P3/00C12R1/645
CPCA01N57/16C07K14/37C12N15/902
Inventor 田世平张占全李博强何畅
Owner INST OF BOTANY CHINESE ACAD OF SCI
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