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Method for efficiently refining crude product heparin sodium

A technology of heparin sodium and crude products, which is applied in the field of heparin sodium production technology, can solve the problems of low yield, large damage to the titer of heparin sodium, and large amount of hydrogen peroxide, etc., and achieve the goals of low yield, increased yield, and increased income Effect

Inactive Publication Date: 2019-03-15
山阳县恒瑞肉制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional production process is to oxidatively decolorize the crude heparin sodium once or twice and refine it by ethanol precipitation. The amount of hydrogen peroxide is large (3-5%), which will greatly damage the titer of heparin sodium and the yield is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The following examples illustrate the invention in detail:

[0020] 1. Crude product dissolution: Add 10 billion units of crude heparin sodium into the precipitation kettle, and fully dissolve it with 600Kg of purified water to obtain 700L of feed solution.

[0021] 2. The first oxidation: adjust the pH to 9.5, adjust the temperature to 15-20°C, add 3.5L of 30% hydrogen peroxide, and oxidize and decolorize for 4 hours under stirring. Afterwards, 2.1 L of 30% hydrogen peroxide was added, and the mixture was oxidized and decolorized for 8 hours while stirring.

[0022] 3. Precipitation for the first time: adjust pH=4.5, add sodium chloride to the feed liquid according to the ratio of 30g / L, and use ethanol (90%) for precipitation at a temperature of 6°C, add while stirring until the ethanol concentration is 35%, Stirring was stopped and allowed to stand for 12 hours.

[0023] 4. The second oxidation: Add the precipitate in 3 to 700L of purified water to fully dissolve, ...

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PUM

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Abstract

The invention relates to a method for efficiently refining crude product heparin sodium. A great number of proteins and nucleic acid impurities are contained in the heparin sodium crude product extracted through the methods, such as salt dissolution, enzyme dissolution and resin adsorption, of pig intestinal mucosa, and refining is carried out by further oxidization sedimentation operation. A onceor twice oxidation sedimentation method is adopted for refining in a traditional technology, and the disadvantages such as not high valence and low yield are extremely difficult to avoid. According to the method, disadvantages of the traditional method are overcome by adopting a repeated gradient oxidation and pH alternate sedimentation method, so that the yield and the unit valence of a heparinsodium competitive product are improved, the economic benefit is increased obviously, and the method has the extremely high practical value.

Description

technical field [0001] The invention relates to a method for refining crude heparin sodium, in particular to a production process for heparin sodium. Background technique [0002] Heparin sodium is a commonly used anticoagulant drug in clinical practice. It is a mucopolysaccharide mainly extracted from pig small intestinal mucosa, which has significant anticoagulant and antithrombotic effects. In recent years, the emergence of low-molecular-weight heparin sodium products has made the application of heparin sodium more and more extensive. The traditional production process is to oxidatively decolorize crude heparin sodium once or twice and refine it by ethanol precipitation. The amount of hydrogen peroxide (3-5%) is large, which greatly damages the titer of heparin sodium and the yield is low. Contents of the invention [0003] The object of the present invention is to provide a high-efficiency process for refining crude heparin sodium, which overcomes the problems in the ...

Claims

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Application Information

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IPC IPC(8): C08B37/10
CPCC08B37/0003C08B37/0075
Inventor 鲁保存
Owner 山阳县恒瑞肉制品有限公司