SNP site of B variant blood type triggering acute hemolytic transfusion reaction

A technology for acute hemolysis and transfusion reactions, applied in recombinant DNA technology, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as misjudgment of blood type, incomplete expression of B antigen, difficulty in detection, etc., and achieve rapid detection , to avoid the effect of acute hemolytic transfusion reaction

Inactive Publication Date: 2019-03-15
QINGDAO BLOOD CENT
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AI Technical Summary

Problems solved by technology

Often due to gene mutations, the expression of B antigen is incomplete and difficult to detect, while the expression of B antibody is often positive, making it easy to miss the B variant, which easily leads to misjudgment of blood type and infusion of unmatched blood or infusion to patients. Uncooperative patients, acute or delayed immune hemolytic reaction

Method used

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  • SNP site of B variant blood type triggering acute hemolytic transfusion reaction
  • SNP site of B variant blood type triggering acute hemolytic transfusion reaction
  • SNP site of B variant blood type triggering acute hemolytic transfusion reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Confirmation of the mutation site of the B variant gene from the genetic testing of the proband of the ABO blood type B variant gene.

[0022] 1. Extraction of peripheral blood genomic DNA:

[0023] In compliance with the relevant national policies and regulations, and on the basis of the consent of the sampling subjects, 2-5ml of peripheral venous blood from members of the ABO blood type B variant gene family was drawn and put into EDTA-Na 2 In anticoagulant tubes, freeze at -80°C for later use; DNA extraction uses the DNA extraction kit from LIFE Company, and the specific operation process is as follows:

[0024] After thawing the frozen EDTA anticoagulated blood at room temperature, take 500ul and put it in a centrifuge tube, add an equal volume of erythrocyte lysate (pH8.0), vortex and mix for 5 minutes, centrifuge at 12000rpm for 5 minutes, and discard the supernatant. Add 500ul of red blood cell lysate repeatedly, vortex and mix for 5 minutes, centrifu...

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Abstract

The invention achieves the purposes by providing an SNP site for detecting the acute hemolytic transfusion reactions induced by B variant type in an ABO blood type system, which is the 586th base froman initiation codon in a novel B variant gene coding region. The invention provides novel application of a B variant gene, and provides an effective approach to rapid genetic diagnosis, genetic screening and genetic counseling of a gene which can trigger the acute intravascular hemolytic transfusion reaction; an application effect indicates that the SNP site of the gene and a detection primer, provided by the invention, can be effectively applied to rapid detection of a novel B variant gene mutation site for peripheral blood of a clinical patient.

Description

technical field [0001] The invention belongs to the technical field of gene diagnosis products, and in particular relates to a SNP site for detecting acute hemolytic transfusion reaction caused by ABO blood group system type B variant blood group gene. Background of the invention [0002] Acute Hemolytic Transfusion Reaction (AHTR) usually occurs within 24 hours after blood transfusion, more often than immediately after blood transfusion. As long as 5-10ml of ABO-incompatible blood is transfused, obvious symptoms will appear; blood transfusion exceeding 200ml will cause serious consequences. Clinically, it often manifests as intravascular hemolysis. Patients with fever, chills, nausea, and back pain, followed by hemoglobinuria, hemoglobinemia, and hypotensive shock, should be considered as acute intravascular hemolytic transfusion reactions, and the blood transfusion should be stopped immediately and administered immediately. Carry out various checks. At the same time, thi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 迟晓云朱于莉韩斌冯智慧
Owner QINGDAO BLOOD CENT
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