Genetic molecular marker co-segregated with male sterility gene msc-2 of capsicum annuum cell nucleus and application of genetic molecular marker

A technology of msc-2msc-2 and cell nucleus, which is applied in the field of gene molecular markers, can solve the problems of long linkage distance and high cost of marker use, and achieve the effects of promoting utilization, reducing breeding and production costs, and saving manpower and material resources

Inactive Publication Date: 2019-03-15
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except for a gene marker developed by msc-1, the rest of the molecular markers linked to the ms gene eit

Method used

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  • Genetic molecular marker co-segregated with male sterility gene msc-2 of capsicum annuum cell nucleus and application of genetic molecular marker
  • Genetic molecular marker co-segregated with male sterility gene msc-2 of capsicum annuum cell nucleus and application of genetic molecular marker
  • Genetic molecular marker co-segregated with male sterility gene msc-2 of capsicum annuum cell nucleus and application of genetic molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1, the acquisition of molecular marker d-CAPs-msc-2

[0061] 1. Development of molecular marker d-CAPs-msc-2

[0062] 1. Construction of positioning groups

[0063] In this experiment, the capsicum nuclear male sterile F 2 Population 17C772 and a male sterile dual-purpose line population 18Q5430 were used as test materials, and a nuclear male sterile F 2 Population 17C772 and a male sterile dual-purpose line population 18Q5430 containing 1034 individual plants were counted for pollen fertility.

[0064] 2. Molecular marker development

[0065] (1) Take the nuclear male sterile F of pepper 2 Fertile individual plants of population 17C772 were mixed to obtain fertile pools. The number of individual plants in each fertile pool is 30.

[0066] (2) Take the nuclear male sterile F of pepper 2 The sterile individual plants of population 17C772 were mixed to obtain sterile pools. The number of single plants in each sterile pool is 25.

[0067] (3) Simplified re...

Embodiment 2

[0093] Example 2, the application of molecular marker d-CAPs-msc-2

[0094] 1. Genomic DNA extraction

[0095] Genomic DNA of 41 pepper homozygous inbred lines or varieties (all fertile) of known genotype (Msc-2Msc-2) shown in Table 1 was extracted.

[0096] Table 1 shows 41 pepper inbred lines and varieties

[0097]

[0098]

[0099] 2. PCR amplification and digestion

[0100] The method of Example 1 was used for PCR amplification and enzyme digestion, and the enzyme digestion products were detected.

[0101] If only contain the fragment that size is 218bp in the restriction product, then described test capsicum genotype is Msc-2Msc-2 (homozygous type), and described test capsicum is homozygous fertile;

[0102] If the digestion product contains fragments with a size of 218bp and 186bp, the genotype of the pepper to be tested is Msc-2msc-2 (heterozygous type), and the pepper to be tested is heterozygous fertile;

[0103] If the digestion product only contains a 186b...

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Abstract

The invention discloses a genetic molecular marker co-segregated with a male sterility gene msc-2 of a capsicum annuum cell nucleus and an application of the genetic molecular marker. Primers for detecting capsicum annuum fertility are provided and are a primer pair capable of amplifying a specific fragment; the specific fragment comprises a target sequence of the primer pair consisting of a primer F and a primer R; the primer F is following a1) or a2): a1) single stranded DNA molecules represented as a sequence 1 of a sequence table; a2) DNA molecules which are obtained by substitution and/ordeletion and/or addition of the sequence 1 by one or more nucleotides and have the same function with the sequence 1; the primer R is following a3) or a4): a3), single stranded DNA molecules represented as a sequence 2 of the sequence table; a2) DNA molecules which are obtained by substitution and/or deletion and/or addition of the sequence 2 by one or more nucleotides and have the same functionwith the sequence 2. Fertile plants can be rapidly identified in the seedling stage according to the developed marker.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a gene molecular marker co-separated with the pepper nuclear male sterility gene msc-2 and an application thereof. Background technique [0002] Pepper (Capsicum annuum L.) is a herbaceous plant belonging to the family Solanaceae (Solanaceae), also known as pepper, sea pepper, spicy eggplant, pepper, etc. According to statistics, the global planting area of ​​pepper has exceeded 50 million mu, with an annual output of 37 million tons. It is the largest seasoning crop in the world. my country's pepper planting area exceeds 24 million mu, accounting for more than 35% of the world's pepper area and about 10% of the country's vegetable area, second only to the cabbage planting area. The total economic output value of pepper exceeds 70 billion yuan, ranking first in terms of output value and benefits. The first vegetable crop (China Vegetable Circulation Association, 2017). P...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 沈火林程青
Owner CHINA AGRI UNIV
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