Protein chip for lung cancer diagnosis and kit

A lung cancer diagnosis and protein chip technology, applied in disease diagnosis, measuring devices, instruments, etc., can solve the problems of invasiveness and high detection cost, inability to use crowd screening, and inconvenient detection of serum samples

Active Publication Date: 2019-03-15
GUANGZHOU BIO BLUE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other detection techniques, such as sputum cytology, fine-needle aspiration cytology, and fluorescent bronchoscopy, all have disadvantages such as high false positive rate, invasiveness, and high detection cost, and ca

Method used

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  • Protein chip for lung cancer diagnosis and kit
  • Protein chip for lung cancer diagnosis and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Embodiment 1 Preparation of the kit containing the protein chip for diagnosis of lung cancer (liquid protein chip diagnostic kit)

[0093] Preparation of antigenic protein chip (liquid chip): purchase commercialized magnetic microbeads from Luminex Company, firstly couple biotin-BSA to magnetic microbeads-COOH through amino coupling reagent, and use the purification and recombination of the 12 kinds of antigens prepared above Proteins (containing Streptavidin tags) bind 12 antigens to the surfaces of magnetic microbeads of different colors through the combination of biotin on the surface of the magnetic microbeads and the Streptavidin tags contained in the antigens. Then add a layer of PBS containing 20% ​​fetal bovine serum on the surface of the magnetic microbeads, place it at room temperature for 1 hour, and then place it overnight at 4°C to fully seal the non-specific protein cross-linking or binding sites on the magnetic microbeads, and finally divide Pack and stor...

Embodiment 2

[0095] Example 2 The kit in Example 1 above was used to diagnose lung cancer and differentiate it from benign pulmonary nodules.

[0096] 200 cases of lung cancer patients and 300 cases of lung benign nodules were detected using the kit described in Example 1 (using protein chip plus immunofluorescence method) to determine the diagnostic criteria for lung cancer. The results are shown in Table 1.

[0097] Table 1

[0098]

[0099] Among 200 cases of lung cancer patients and 300 cases of lung benign nodules, the kit detected 142 positive cases of lung cancer patients and 27 cases of positive lung benign nodules. Therefore, the sensitivity of the kit for diagnosing lung cancer was 71.0%. The specificity of the differential diagnosis of benign pulmonary nodules was 91.0%, indicating that the sensitivity of the kit for the diagnosis of lung cancer and the specificity of the differential diagnosis of benign pulmonary nodules were high.

[0100] Among the 200 cases of lung canc...

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Abstract

The invention relates to a protein chip for lung cancer diagnosis and a kit. The protein chip for the lung cancer diagnosis comprises a solid phase supporting matter and an antigen which is wrapped with the surface of the solid phase supporting matter, wherein the antigen comprises the following twelve antigen fragments: a Paxi11in antigen fragment, a CTAG1B antigen fragment, an SOX2 antigen fragment, an HSPA9 antigen fragment, a UBQLN1 antigen fragment, a C14orf104 antigen fragment, an ENO-1 antigen fragment, a RPS3 antigen fragment, a CHK antigen fragment, an HNRPA1 antigen fragment, a PCNAantigen fragment and an Eif4g3 antigen fragment. According to the protein chip and the kit, the antigens which are obviously expressed in the early lung cancer and are capable of enabling related antibody growing in the immune system are creatively selected, and the antigens are not expressed or expressed a little in non-cancer benign lesion; the screened antigen fragments are combined to use; theprotein chip technology is carried out to detect the own antibody, related to tumor, in human serum and identify benign nodule of early lung cancer and the lung; and the sensitivity and the specificity are high.

Description

technical field [0001] The invention relates to the field of cancer diagnosis and treatment, in particular to a protein chip and a kit for lung cancer diagnosis. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. Currently, the diagnosis of lung cancer mainly relies on imaging (such as spiral CT) and cytology. Spiral CT can detect lung lesions more than 3nm more sensitively, but it has a high false positive rate for the diagnosis of lung cancer, especially for the identification of early lung cancer and benign pulmonary nodules. Other detection techniques, such as sputum cytology, fine-needle aspiration cytology, and fluorescent bronchoscopy, all have the disadvantages of high false positive rate, invasivenes...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57423G01N33/57488G01N2800/60
Inventor 胡海许勇林当周海鹰黄璐潘敬东
Owner GUANGZHOU BIO BLUE TECH CO LTD
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