Compound biological agent for preventing and controlling sesame damping-off and application of compound biological agent
A technology of blight and sesame, which is applied in the field of compound biological agents for the prevention and control of sesame blight, can solve problems such as unsatisfactory control effects and serious environmental hazards, achieve significant control and control effects, increase yields, and improve Effects on people's living standards
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Embodiment 1
[0023] Embodiment 1, compounding and screening of indoor biological bacteria agent
[0024] 1. Zone of inhibition experiment
[0025] The pathogenic bacteria Rhizoctonia solani (Rhizoctonia solani) was isolated from the pathogenic part of sesame blight, and a plate containing bacteria was made, and Bacillus subtilis (A), Pseudomonas fluorescens (B) and Agrobacterium radioactive ( C) bacteria suspension (the number of effective viable bacteria is 1 × 10 9 CFU / L, suspended in sterile water) were mixed according to different volume ratios to prepare different medicinal solutions, dipped in sterile filter paper pieces (diameter 0.6cm) and placed on bacteria-containing plates, 3 pieces per dish , with sterilized water and binary compound drug solution as the control respectively, repeated 3 times, and measured the diameter of the inhibition zone after culturing at 28°C for 48 hours. The results are shown in Table 1.
[0026] Table 1. Inhibitory effects of different medicinal sol...
Embodiment 2
[0051] Embodiment 2, the biological control of sesame blight in the field
[0052] The experiment was set up in the plots where sesame blight had occurred over the years in Shandong Province, and the cultivation conditions (soil type, fertilization, sowing and planting period, growth stage, and row spacing) of each experimental plot were uniform. There were 7 treatments in the experiment, and each treatment was repeated 3 times. The experiment was arranged in random blocks, and the area of the plot was 50m 2 . Precise soil preparation and high border cultivation, record the diseased plants in each experimental plot from one month after sowing the treated seeds to before harvesting new sesame, and finally count the incidence of disease and sesame yield throughout the growth period to calculate the control effect. The results are shown in Table 3.
[0053] Incidence rate = (number of diseased plants / total number of investigated plants) × 100%;
[0054] Control effect=[(incide...
Embodiment 3
[0064] Embodiment 3, the biological control of sesame blight in the field
[0065] The experiment was set up in the plots where sesame blight had occurred over the years in Shandong Province, and the cultivation conditions (soil type, fertilization, sowing and planting period, growth stage, and row spacing) of each experimental plot were uniform. Precise soil preparation and high border cultivation, the full and uniform sesame seeds are sown and treated 20 days after emergence as follows:
[0066] Treatment 1: Use A, B, and C volume ratios in Table 2 to irrigate the roots, with an average dosage of 0.2 liters per plant;
[0067] Treatment 2: Use A, B, and C in Table 2 to irrigate the roots with a medicinal solution whose volume ratio is 1:4:2, with an average dosage of 0.2 liters per plant;
[0068] Treatment 3: Use A, B, and C in Table 2 with a volume ratio of 1:4:4 to irrigate the roots, with an average dosage of 0.2 liters per plant;
[0069] Treatment 4: Use 40% thiram r...
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