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DNA (deoxyribonucleic acid) dye compound with nuclear targeting function and application

A dye compound, cell nucleus technology, applied in the direction of organic dyes, azo dyes, analytical materials, etc., can solve the problems of inability to achieve, physiological process disorder, cell damage, etc., and achieve the effect of excellent cell nucleus targeting

Active Publication Date: 2019-03-19
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hoechst and DAPI are two dyes that can bind to DNA, but the excitation light wavelength of Hoechst and DAPI is relatively short, and the cells will be affected by phototoxicity during the imaging process, which will cause normal physiological process disorder or cause serious cell damage, and Hoechst and DAPI can only target the nucleus but cannot achieve other functions, such as pH measurement, etc.

Method used

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  • DNA (deoxyribonucleic acid) dye compound with nuclear targeting function and application
  • DNA (deoxyribonucleic acid) dye compound with nuclear targeting function and application
  • DNA (deoxyribonucleic acid) dye compound with nuclear targeting function and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026]

[0027] Under the protection of argon, compound A1 (50mg, 98.50umol) and compound B1 (55mg, 118.21umol) were added to the reaction flask, anhydrous copper sulfate aqueous solution, sodium ascorbate aqueous solution and 4 drops of N,N-diisopropyl Ethylamine was reacted at room temperature for 24h, and the solvent was evaporated under reduced pressure. The crude product was separated by column chromatography (distillate CH2Cl2:MeOH=8:1) to obtain a purple solid (60mg, 62.04umol, 62.98%). The product structure was identified by HRMS, HRMS[M+H] + : 967.4164.

Embodiment 2

[0029]

[0030] The synthetic method refers to Example 1, replaces B1 with compound B2, and the product structure is identified by HRMS, HRMS [M+H] + : 963.3864.

Embodiment 3

[0032]

[0033] The synthetic method refers to Example 1, replaces B1 with compound B3, and the product structure is identified by HRMS, HRMS [M+H] + : 1019.4374.

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Abstract

The invention discloses a DNA (deoxyribonucleic acid) dye compound with a nuclear targeting function and an application, and belongs to the field of fine chemical engineering. In the field of bioimaging, visible research of genetic materials is of great significance for cellular activity research. The wavelength of exciting light needed by dyes Hoechst and DAPI of traditional targeting cell nucleuses is short, and serious cellular damage is caused in imaging. In addition, the Hoechst and the DAPI can only purely target the cell nucleuses, but cannot achieve other functions. In order not to affect normal physiological functions of cells, the DNA dye compound has high biocompatibility, can be specifically combined with DNA and has the nuclear targeting function.

Description

technical field [0001] The invention relates to a class of DNA dye compounds with cell nucleus targeting function and applications thereof, in particular to a class of dye compounds used for cell nucleus targeting, which belongs to the field of fine chemicals. Background technique [0002] The nucleus is the main organelle for eukaryotic cells to maintain gene integrity and regulate cell functions, and contains a large amount of genetic material, namely DNA. Each eukaryotic cell contains about two meters of DNA, which interacts with proteins to form highly structured chromatin. DNA is mainly present in the nucleus in the form of chromatin or chromosomes. The regulation of cell function by the nucleus is mainly achieved through the activities of regulatory DNA. Therefore, the study of highly structured chromatin or chromosomes in the nucleus is of great significance to the study of the nucleus and the control of cell life activities. [0003] In the field of biological ima...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B57/00C09K11/06G01N21/64
CPCC09B57/00C09K11/06C09K2211/1059C09K2211/1088G01N21/6402
Inventor 肖义张晓东张新富
Owner DALIAN UNIV OF TECH
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