Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Tissue culture method for succulent adromischus marianiae hallii

A technology for tissue culture and succulent plants, applied in the fields of botany equipment and methods, plant regeneration, gardening methods, etc., can solve the problems of inapplicability

Active Publication Date: 2019-03-22
LINYI UNIVERSITY
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the tissue culture method discloses the types and dosages of the selected phytohormones at different stages by inducing callus culture and adventitious bud induction culture, the absorption and growth of different succulents to different types of phytohormones are different, so , this succulent plant tissue culture method is not applicable to the tissue culture of all kinds of succulent plants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture method for succulent adromischus marianiae hallii
  • Tissue culture method for succulent adromischus marianiae hallii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1. Disinfection of explants:

[0066] Leaf explants soaked in red eggs: take the leaves soaked in red eggs as explants, wash the surface of each leaf with 0.2g of washing powder, and then wash with tap water for 5 minutes to remove surface dirt, and place the explants on an ultra-clean workbench superior. Before inoculation, put the washed red egg blister leaves into a sterilized Erlenmeyer flask, and then add 0.1% HgCl with a volume concentration of 0.1% Tween-20 2 The surface of the solution was disinfected for 3-4 minutes, washed with sterile water for 5 times, each time for 1 minute, and the sterilized explants were soaked in sterile water for 1 minute, and then the sterile water was replaced. Then the washed explants were placed in a sterilized stainless steel dish, and the moisture on the surface of the explants was air-dried for later use.

[0067] 2. Callus induction:

[0068] Stain a little carbenicillin powder on the broken part of the surface-sterilized le...

Embodiment 2

[0085] 1. Disinfection of explants:

[0086] Leaf explants soaked in red eggs: take the leaves soaked in red eggs as explants, wash the surface of each leaf with 0.2g of washing powder, and then wash with tap water for 5 minutes to remove surface dirt, and place the explants on an ultra-clean workbench superior. Before inoculation, put the washed red egg blister leaves into a sterilized Erlenmeyer flask, and then add 0.05% Tween-20 with 0.1% HgCl 2 The surface of the solution was disinfected for 3-4 minutes, washed with sterile water for 5 times, each time for 1 minute, and the sterilized explants were soaked in sterile water for 1 minute, and then the sterile water was replaced. Then the washed explants were placed in a sterilized stainless steel dish, and the moisture on the surface of the explants was air-dried for later use.

[0087] 2. Callus induction:

[0088] Stain a little carbenicillin powder on the broken part of the surface-sterilized leaf explants, inoculate th...

Embodiment 3

[0105] The red egg vesicles were cultivated according to the method of Example 2, except that the hormone content in the induction medium selected for the induction and proliferation of the green spherical single particles in step 3 was zeatin 1.5 mg / L and 0.5 mg / LIAA. The results showed that the number of spherical particles obtained in the culture medium of 1.5mg / L zeatin combined with 0.5mg / L IAA was 60 / dish.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a tissue culture method for succulent adromischus marianiae hallii, belonging to the technical field of sedum succulent tissue culture methods. According to the tissue culture method for the succulent adromischus marianiae hallii, the leaves of the adromischus marianiae hallii are used as explants, the steps including callus induction, green spherical particle induction, green spherical particle hardening and seedling strengthening, domestication and transplanting, etc. are performed to obtain a large number of culture seedlings. The tissue culture method provided by theinvention improves the regeneration frequency of the adromischus marianiae hallii leaves, the reproduction coefficient is as high as 200 times or more, and the survival rate of the domestication andtransplanting is high, which reaches 90 percent or more. The reproduction efficiency of the adromischus marianiae hallii can be improved compared with the common leaf insertion propagation method, which provides technical support for the large-scale production of the adromischus marianiae hallii, and has a certain reference value for the research of other adromischus succulent, and is a adromischus marianiae hallii breeding method with obvious economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of succulent plant tissue culture methods, and in particular relates to a tissue culture method of succulent plant red egg blisters. Background technique [0002] Red Egg Blister (Adromischus marianiae'hallii') is a succulent plant of the Sedum genus (Blister) of Crassulaceae, produced in the Karoo Plateau of South Africa, and is a cultivar of Marian Adromischus marianiae. Red egg blisters are small succulent plants with round or broad oval leaves, thin edges and thick middle, ring-shaped clusters on the stem, fine texture on the leaf surface, green to red leaf color. The red eggs are soaked in water, the leaves are fat and swollen, the red edges of the leaves are obvious, and the leaves are reddish, quite cute. There are not many varieties bred under the red egg blister line, but almost every variety has become a classic. [0003] The red egg blister plant is a perennial succulent herb. The red egg bliste...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 廉玉姬林光哲
Owner LINYI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com