Polypeptide with vitiligo virus inhibitory effect and its application
A vitiligo syndrome and virus suppression technology, applied in antiviral agents, medical preparations containing active ingredients, peptides, etc., can solve the problems of chemical disinfection residues, lack of drugs, etc. Effect
Active Publication Date: 2022-02-08
QINGDAO UNIV OF SCI & TECH
View PDF4 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0009] The purpose of the present invention is to solve the problems of lack of ESSV control drugs and residues in chemical disinfection, and provide a polypeptide with an inhibitory effect on white spot syndrome virus. This polypeptide is used for the development of WSSV inhibitory drugs and has the advantages of no residue and no side effects
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0025] (1) Peptide synthesis: Using the Hydropathy analysis provided by the MPEx server, a peptide with potential inhibitory effect was obtained: Ser Ala Ile Leu Ala Ile Thr Ala Val Ile Ala Val Phe Ile Val Ile PheArg Tyr; commissioned by Nanjing Jiepei Biotechnology Co., Ltd. The chemical synthesis was carried out by solid-phase synthesis. The amino and carboxyl ends are not modified. After synthesis, it is purified by HPLC with a purity of 95.86%, and identified by mass spectrometry. The results are as follows: figure 1 shown.
[0026] (2) Control experiment: the challenge control group and the challenge experimental group were set up respectively, with 10 adult shrimps in each group, and the prawn species was Litopenaeus vannamei; the synthesized polypeptide was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 0.5 mg / mL; each shrimp in the challenge control group was injected with 40 microliters of WSSV virus suspension, and the challenge experimental group was...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
On the one hand, the present invention provides a polypeptide having a white spot syndrome virus inhibitory effect, the amino acid sequence of which is shown in SEQ ID NO: 1, which is Ser Ala Ile Leu Ala Ile Thr Ala Val Ile Ala Val Phe Ile Val Ile Phe Arg Tyr; Its amino acid sequence is either a functional equivalent of SEQ ID NO: 1, said functional equivalent being an amino-terminal or carboxy-terminal modified sequence of SEQ ID NO: 1, a sequence in which D-amino acids are substituted for L-amino acids, a sequence in which amino acids are deleted or added . The present invention also provides the application of the above polypeptide in the preparation of medicines for inhibiting white spot syndrome virus. The polypeptide obtained by the present invention has small molecular weight, easy synthesis, simple structure, no residue, no side effects, and has inhibitory effect on the invasion of prawns by white spot syndrome virus, and is especially suitable for the protection of prawns in the early stage of virus outbreak. It is of great value in the development of anti-virus drugs.
Description
technical field [0001] The invention relates to a polypeptide having an inhibitory effect on white spot syndrome virus, and belongs to the field of biochemistry and biotechnology. Background technique [0002] White spot syndrome virus (WSSV) is the main pathogenic microorganism that causes mass mortality of prawns and seriously threatens the development of prawn aquaculture. In order to prevent and control WSSV infection, researchers have worked hard, but there is still no effective control method so far. At present, it is mainly prevented by breeding disease-resistant shrimp species, strengthening disease monitoring, controlling stocking density and regular disinfection. White spot syndrome Prevention and treatment still have a long way to go. [0003] In recent years, studies have found that shrimp also has specific immune phenomena similar to vertebrates, and people have begun research on the immune prevention of shrimp white spot syndrome. Vaccines made of recombinant...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/20
CPCA61P31/20C07K7/08A61K38/00
Inventor 卢永忠邱倩刘杰程琳越
Owner QINGDAO UNIV OF SCI & TECH