A method for detecting microorganisms in wood

A microbial and wood technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as difficult to purify strains, limit microbial research, and microbial identification

Active Publication Date: 2021-08-31
INST OF WOOD INDUDTRY CHINESE ACAD OF FORESTRY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many microorganisms are inseparable from the special environment in which they live, and are difficult to isolate. Only 1% to 5% of microorganisms can be purely cultured, and because the growth speed of microorganisms is different in the process of tissue isolation and cult...

Method used

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  • A method for detecting microorganisms in wood
  • A method for detecting microorganisms in wood
  • A method for detecting microorganisms in wood

Examples

Experimental program
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Embodiment 1

[0045] Embodiment 1, a kind of method for detecting microorganisms in wood

[0046] Three pieces of microbial spoilage and discoloration from different color changes (that is, wood decay and discoloration caused by microorganisms) are recorded as A, B and C ( figure 1 ) were treated according to the following methods, and these three woods all had typical biodegradation symptoms of discoloration:

[0047] 1. Collection of wood sawdust

[0048] The wood is pretreated in a constant temperature and humidity box, and the moisture content of the wood is adjusted to about 20% to obtain the pretreated wood; the surface of the pretreated wood is sterilized with 75% ethanol aqueous solution to obtain the sterilized wood ;Select the position with typical biological deterioration symptoms in the sterilized wood, drill three positions with a hexagonal drill with a diameter of 5mm, collect the generated sawdust, and then collect each 0.1g sawdust as a portion and place it in a 1ml centrif...

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Abstract

The invention discloses a method for detecting microorganisms in wood. The method for detecting microorganisms in wood of the present invention first needs to pretreat the wood in a constant temperature and humidity box to keep the moisture content at about 20%, and then after the surface is sterilized, the sawdust is drilled quantitatively and at a certain depth, and after adding β-mercaptoethanol The dodecyltrimethylammonium bromide (DTAB) solution was cracked to extract the total microbial genetic material in the wood tissue cells, and then the microbial information in the wood was obtained through amplification and sequencing, and the microbial species in the wood were determined. Compared with the traditional separation method, the method of the present invention can obtain more complete wood microbial information, which is beneficial to the effective prevention and control of wood decay or the degradation of wood, and effectively makes up for the fact that the current detection of wood microbes cannot be separated from separation, purification and The shortcomings of the traditional method of tissue culture provide a new test method for the biodegradation and control of wood.

Description

technical field [0001] The invention belongs to the field of wood protection and relates to a method for detecting microorganisms in wood. Background technique [0002] Microbial decay of wood is the main cause of wood degradation and recycling in nature, and an indispensable part of maintaining the carbon and nitrogen cycle in nature, but it is one of the problems that need to be faced and solved in wood processing and utilization. The main types of microbial spoilage are mildew, discoloration, etching, soft rot and decay. Microbial factors mainly refer to bacterial and fungal factors. Detect wood microbial hazards, judge the degree of wood hazards, understand the microbial factors that cause wood, and make prevention and control targeted. Bacterial hazards, mold hazards, discoloration fungus hazards, and decay fungus hazards require different control methods. Because wood decay first begins in the interior or in hard-to-reach areas such as the ground, it is difficult to...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/04
CPCC12Q1/6869C12Q2531/113
Inventor 马星霞张斌张景朋蒋明亮吴玉章屈伟
Owner INST OF WOOD INDUDTRY CHINESE ACAD OF FORESTRY
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