A method for detecting microorganisms in wood

Abstract

The invention discloses a method for detecting microorganisms in wood. The method for detecting microorganisms in wood of the present invention first needs to pretreat the wood in a constant temperature and humidity box to keep the moisture content at about 20%, and then after the surface is sterilized, the sawdust is drilled quantitatively and at a certain depth, and after adding β-mercaptoethanol The dodecyltrimethylammonium bromide (DTAB) solution was cracked to extract the total microbial genetic material in the wood tissue cells, and then the microbial information in the wood was obtained through amplification and sequencing, and the microbial species in the wood were determined. Compared with the traditional separation method, the method of the present invention can obtain more complete wood microbial information, which is beneficial to the effective prevention and control of wood decay or the degradation of wood, and effectively makes up for the fact that the current detection of wood microbes cannot be separated from separation, purification and The shortcomings of the traditional method of tissue culture provide a new test method for the biodegradation and control of wood.

Description

technical field [0001] The invention belongs to the field of wood protection and relates to a method for detecting microorganisms in wood. Background technique [0002] Microbial decay of wood is the main cause of wood degradation and recycling in nature, and an indispensable part of maintaining the carbon and nitrogen cycle in nature, but it is one of the problems that need to be faced and solved in wood processing and utilization. The main types of microbial spoilage are mildew, discoloration, etching, soft rot and decay. Microbial factors mainly refer to bacterial and fungal factors. Detect wood microbial hazards, judge the degree of wood hazards, understand the microbial factors that cause wood, and make prevention and control targeted. Bacterial hazards, mold hazards, discoloration fungus hazards, and decay fungus hazards require different control methods. Because wood decay first begins in the interior or in hard-to-reach areas such as the ground, it is difficult to...

AI Technical Summary

Problems solved by technology

However, many microorganisms are inseparable from the special environment in which they live, and are difficult to isolate. Only 1% to 5% of microorganisms can be purely cultured, and because the growth speed of microorganisms is different in the process of tissue isolation and culture, microorganisms that grow too fast may cover up Slow-growing microorganisms make it difficult to purify strains, which leads to biased results. Even if many microorganisms are isolated, it is difficult to identify them by morphology, which greatly limits the research of microorganisms

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