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Application of leech saliva to preparation of analgesic drugs

The technology of leech and medicine is applied in the application field of leech saliva in the preparation of analgesic medicines, which can solve the problems of urgent development of medicinal value, influence on people's lives, small side effects and the like, and achieve the effect of good analgesic effect.

Pending Publication Date: 2019-03-29
KUNMING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Pain is the body's defense response. It reflects the body's problems, and it can be cured in time to keep away from pain. However, the pain response in real life has seriously affected people's lives.
There are many types of analgesics, and it is convenient for people to buy them, but the side effects of analgesics should not be underestimated. Analgesics with traditional Chinese medicine as the main component may have better analgesic effects and less side effects
[0005] Current research on leeches mainly focuses on antithrombotic activity, and its analgesic medicinal value is urgently to be developed

Method used

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  • Application of leech saliva to preparation of analgesic drugs
  • Application of leech saliva to preparation of analgesic drugs
  • Application of leech saliva to preparation of analgesic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Obtain leech saliva

[0017] Use a 6V, 2.5-345Hz multi-functional electromagnetic massager to stick to the corners of the mouth on both sides of Hirudo phenanthae for 5-10s to stimulate. Visible colorless viscous liquid overflows from the mouth. Use saliva stimulation liquid (150mM NaCl, 1mM L-Arginine ) was washed repeatedly, the washing solution was collected, centrifuged (4° C., 12000 g, 1 h), and vacuum freeze-dried to obtain a freeze-dried powder.

[0018] Dissolving the above lyophilized powder with deionized water, using a dialysis bag to remove NaCl and L-Arginine therein, and vacuum lyophilizing the obtained protein solution again to obtain leech saliva lyophilized powder. The obtained leech saliva freeze-dried powder was dissolved with physiological saline to obtain a mother solution with a concentration of 10 mg / mL.

Embodiment 2

[0019] Embodiment 2: Mouse writhing pain experiment caused by acetic acid

[0020] The mother liquor obtained in Example 1 was diluted with physiological saline, and sterilized with a 0.22 μm filter.

[0021] Get 50 Kunming mice, be divided into 5 groups at random, namely: normal saline group, aspirin group 20mg / Kg, leech saliva high-dose group (10mg / Kg), middle-dose group (2mg / Kg), low-dose group (0.4mg / Kg), 10 per group. The administration method was tail vein injection, and 0.2 ml / mouse of 0.5% acetic acid solution was intraperitoneally injected into the mice of each group 15 minutes after the administration. Then, abdominal invagination, limb extension and buttock elevation were used as pain response indicators, the number of writhing reactions within 30 minutes was observed, the differences between groups were compared, and the percentage of drug analgesia was calculated. Analgesic rate% = (number of writhing times in the normal saline group - number of writhing times ...

Embodiment 3

[0027] Example 3: Hot plate labor pain test

[0028] Female mice were placed on a metal hot plate at (55±0.5)°C, and the mice whose pain threshold was 15-30s were selected, and randomly divided into normal saline group, morphine group 2 mg / Kg, and leech saliva high-dose group ( 10mg / Kg), middle dose group (2mg / Kg), low dose group (0.4mg / Kg), 10 rats in each group. Mice were injected into the tail vein, and the pain threshold was measured at different times after the administration. The increase in the pain threshold after the drug was used as an index for comparison between groups. The results are shown in Table 2.

[0029] Table 2 Analgesic results of leech saliva hot plate method

[0030]

[0031] Example 1 The hot plate analgesic experiment proved that leech saliva has a better analgesic effect on heat pain, and within the detection time, the analgesic effect first increased and then weakened.

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Abstract

The invention relates to the technical field of analgesic drugs, in particular to an application of leech saliva to preparation of analgesic drugs. The leech saliva is collected by stimulation of electricity and a stimulation liquid, the treated leech saliva is injected into mouse bodies intravenously, and the leech saliva is proved to have good analgesic activity through a mouse writhing experiment, a hot plate analgesia experiment and a skin trauma model experiment which are caused by acetic acid, and the analgesic effect is enhanced with increase of dosage. According to the phenomenon thatleeches bite people without pain, it is discovered that the leech saliva has analgesic effect, and the application of the leech saliva in preparation of the analgesic drugs is provided.

Description

technical field [0001] The invention relates to the technical field of analgesic drugs, in particular to the application of leech saliva in preparing analgesic drugs. Background technique [0002] Leeches, commonly known as leeches, have been recorded in "Shen Nong's Materia Medica", and have high medicinal value; they grow and reproduce in inland freshwater waters, and are traditional Chinese special medicinal aquatic animals. Used as medicine, it has the effects of treating stroke, high blood pressure, clearing blood stasis, amenorrhea, and traumatic injuries. In recent years, it has been newly discovered that leech preparations have special effects in the prevention and treatment of cardiovascular and cerebrovascular diseases and cancer. Historically, it was mainly caught by natural fishing. Due to the abuse of pesticides and chemical fertilizers in recent years, and the pollution of the environment by the "three wastes" of industry and agriculture, the wild natural reso...

Claims

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Application Information

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IPC IPC(8): A61K35/62A61P29/00A61P25/00
CPCA61P25/00A61P29/00A61K35/62
Inventor 刘子超杜新茹祝平袁永生仝向荣
Owner KUNMING UNIV
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