Cell screening method and system based on immunomagnetic bead labeling

An immunomagnetic bead and screening method technology, which is applied in the field of cell screening methods and systems based on immunomagnetic bead labeling, can solve problems such as difficulty in ensuring the successful capture of trace target cells, difficulty in ensuring successful capture of cells, and inability to meet informatization requirements, etc. Achieve the effect of realizing signal-to-noise ratio magnetic cell detection, high signal-to-noise ratio magnetic cell detection, and meeting the needs of informatization

Active Publication Date: 2022-04-29
太锐芯科技(青岛)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This protocol can remove other cells and unbound magnetic beads, but it is difficult to ensure that trace target cells are successfully captured
Moreover, this solution uses a chemical combination method, which is difficult to ensure the successful capture of very small cells such as circulating tumor cells, which will cause misdiagnosis in diagnosis
In addition, this method of capturing target cells through antibody-antigen binding requires manual participation and must be completed by professional technicians, which is not conducive to integration and cannot meet the informatization needs of the big data era

Method used

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  • Cell screening method and system based on immunomagnetic bead labeling
  • Cell screening method and system based on immunomagnetic bead labeling
  • Cell screening method and system based on immunomagnetic bead labeling

Examples

Experimental program
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Embodiment 1

[0041] The cell screening method based on immunomagnetic bead labeling of the present invention includes, as figure 1 As shown in the cell screening device, the cell screening device includes a hollow cuboid shell, and a layer of cell sieves is arranged horizontally inside the shell to divide the shell into an upper cavity and a lower cavity, and the cavity is filled with phosphate buffer saline PBS . The left side of the cell screening device is provided with a sample inlet corresponding to the lower cavity; the right side of the cell screening device is respectively provided with an upper sample outlet and a lower sample outlet corresponding to the upper cavity and the lower cavity.

[0042] In this embodiment, setting the housing of the cell screening device in the shape of a cuboid is only a preferred form. Of course, the housing of the cell screening device can be in a regular shape such as a cylinder, a cube, or any irregular shape. Those skilled in the art can make spe...

Embodiment 2

[0061] The difference between embodiment 2 and embodiment 1 is that in this embodiment, transverse oscillation microwaves are selected for transverse oscillation, which are generated by microwave generators. Under the force of transverse microwave, the immunomagnetic beads shake slightly, and the speed and pass rate of the immunomagnetic beads passing through the cell sieve are very high.

Embodiment 3

[0063] The difference between embodiment 3 and embodiment 1 lies in that in this embodiment, lateral alternating water flow is selected for lateral oscillation. The immunomagnetic beads shake slightly under the force of the lateral alternating water flow, and the speed and pass rate of the immunomagnetic beads passing through the cell sieve are very high.

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Abstract

The invention belongs to the cross field of cell sorting technology and biomedical magnetic sensor technology, and specifically relates to a cell screening method and system based on immunomagnetic bead labeling, including a cell screening device, the main structure of which is covered with a layer of cells Screening is divided into an upper layer and a lower chamber, and the chamber is filled with a cell buffer; the screening method includes the following steps, S1. will contain free immunomagnetic beads, target cells marked by immunomagnetic beads and other The cell sample is input into the lower chamber of the cell screening device; S2. Apply a longitudinal magnetic field and transverse oscillation to the cell screening device, suck the immunomagnetic beads into the upper chamber, and the target cells are adsorbed on the lower surface of the cell screen; S3. Wash separately A solution containing free immunomagnetic beads, a solution containing other cells, and a solution containing target cells labeled with immunomagnetic beads are obtained. The method adopts a physical method to realize cell screening, which can greatly improve the capture rate of target cells, and the device used to complete the method of the present invention can easily integrate sensors to meet the informatization needs of the big data era.

Description

technical field [0001] The invention belongs to the intersection field of cell sorting technology and biomedical magnetic sensor technology, and specifically relates to a cell screening method and system based on immunomagnetic bead labeling. Background technique [0002] Immunomagnetic beads have been widely used in molecular and cell biology, including nucleic acid extraction, sorting and enrichment of specific proteins and cells, etc. Taking immunomagnetic bead-based cell sorting as an example, the process is divided into four steps: (1) surface modification of magnetic beads with specific ligands or antibodies; (2) passing the modified magnetic beads through antigen- The antibody reaction or the ligand-receptor binding reaction binds to the target cells in the sample; (3) magnetically adsorbs the magnetic bead-cell conjugate; (4) removes other cells by washing and other means. [0003] The existing cell screening technology based on immunomagnetic beads directly attract...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00C12N13/00C12M1/00C12M1/42
CPCC12M23/02C12M35/06C12N5/00C12N13/00
Inventor 温良恭李智
Owner 太锐芯科技(青岛)有限公司
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