Preparation method and application of oat beta-dextran with small and medium molecular weight
A low-molecular-weight, glucan technology, which is applied in medical preparations containing active ingredients, skin care preparations, pharmaceutical formulations, etc., can solve the problems of low yield of oat β-glucan, increase production, avoid The effect of wasting resources
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Embodiment 1
[0066] The enzymatic hydrolysis method is as follows: use water as the carrier, use commercially available oat β-glucan powder with a content of 70% as the substrate raw material, adjust the concentration of the substrate (namely oat β-glucan) to 5%, and use enzyme 1 as the Hydrolase; adjust the enzyme-to-substrate ratio (enzyme:substrate mass ratio) to 1:1000; adjust the pH to 4.5; enzymolysis temperature to 45°C; enzymolysis time to 0.5h. After enzymolysis, carry out molecular weight classification. The molecular weight classification method is: use ultrafiltration membrane to divide the molecular weight into parts with a molecular weight greater than 30000 Daltons and parts with a molecular weight less than 30000 Daltons. Step 3 times, combine oat β-glucan solutions less than 30,000 Daltons, heat to 95°C to inactivate enzymes for 30 minutes, remove protein by isoelectric point method (conventional technology in this field), and concentrate to β-glucan content The 2.5% solut...
Embodiment 2
[0068] The enzymatic hydrolysis method is as follows: use water as the carrier, use oat bran as the substrate raw material, adjust the concentration of the substrate (ie oat β-glucan) to 1%, use enzyme 2 as the hydrolytic enzyme; adjust the enzyme-to-substrate ratio to 1: 5000; adjust the pH to 5.0; enzymatic hydrolysis temperature is 50°C; each enzymatic hydrolysis time is 1h. After enzymatic hydrolysis, carry out molecular weight classification. The molecular weight classification method is: use ultrafiltration membrane to divide the molecular weight into parts with a molecular weight greater than 10,000 Daltons and those with a molecular weight less than 10,000 Daltons. Twice, combine oat β-glucan solutions less than 10,000 Daltons, heat to 95°C to inactivate enzymes for 30 minutes, remove protein by flocculation, concentrate to a solution with β-glucan content greater than 2.5%, add deionized Water and butanediol are prepared into a solution containing 8% butylene glycol a...
Embodiment 3
[0070]The enzymatic hydrolysis method is as follows: use water as the carrier, use commercially available oat β-glucan powder with a content of 70% as the substrate raw material, adjust the concentration of the substrate (namely oat β-glucan) to 10%, and use enzyme 2 as the Hydrolase; adjust the enzyme-to-substrate ratio to 1:100; adjust the pH to 9.0, enzymatic hydrolysis temperature to 80°C, and each enzymatic hydrolysis time to 0.1h. After enzymatic hydrolysis, carry out molecular weight classification. The molecular weight classification method is: use molecular sieve method to divide the molecular weight into parts with a molecular weight greater than 1000 Daltons and those with a molecular weight less than 1000 Daltons. 1 time, combine oat β-glucan solutions less than 1000 Daltons, heat to 95°C to inactivate enzymes for 30 minutes, remove protein by flocculation, concentrate to a solution with β-glucan content greater than or equal to 0.5%, and freeze-dry , to obtain oat...
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