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Detection reagent and method for urinary and salivary iodine via microplate reader after normal-temperature pretreatment

A microplate reader and pretreatment technology, applied in the field of medical testing, can solve the problems of increasing steps and equipment costs, explosion of chloric acid and potassium chlorate, and increasing difficulty, so as to shorten the sampling time, reduce human error, and small error value Effect

Inactive Publication Date: 2019-03-29
王九宏
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the chloric acid digestion method effectively eliminates the potential interference of interfering substances and makes the determination of urinary iodine more accurate, there are still shortcomings in this method.
First, chloric acid is difficult to buy directly from the supplier, and it needs to be produced in the laboratory by using the reaction of perchloric acid and potassium chlorate, which increases the difficulty of the experiment
Second, the sample needs to be heated during digestion, which increases the experimental steps and equipment costs
Third, chloric acid and potassium chlorate are potentially explosive

Method used

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Embodiment Construction

[0033] The specific embodiment of the invention will be described in further detail below.

[0034] Urine iodine and saliva iodine of the present invention are pre-treated at normal temperature, and the reagents for microplate detection are made up of the following components:

[0035] (1), A reagent: sodium hypochlorite solution NaClO M r =74.44, 0.5~2.0mol / L;

[0036] (2), B reagent: sulfuric acid solution H 2 SO 4 m r =98.078, 0.5~2.0mol / L;

[0037] (3), C reagent: arsenous acid solution H 3 AsO 3 , 0.01~0.2mol / L;

[0038] (4), D reagent: cerium ammonium sulfate solution Ce(NH 4 ) 4 (SO 4 ) 4 2H 2 O, M r =632.6, 0.01~0.2mol / L;

[0039] (5), calibration solution: iodine standard series solution (potassium iodate solution KIO 3 ,M r = 214.0).

[0040] The number of the calibration solutions is seven, and their concentrations are: 0 μg / L, 25 μg / L, 50 μg / L, 100 μg / L, 200 μg / L, 400 μg / L, 600 μg / L.

[0041] The reagents and detection methods for the detection o...

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Abstract

The invention discloses a detection agent for urinary and salivary iodine via a microplate reader after normal-temperature pretreatment. The agent is characterized by comprising an agent A, a 0.5-2.2mol / L of sodium hypochlorite solution NaClO in which Mr=74.44; an agent B, a 0.5-2.2mol / L of sulphuric acid solution H2SO4 in which Mr=98.078; an agent C, a0.01-0.2mol / L of arsenous acid solution H3AsO3; an agent D, a 0.01-0.2mol / L of ammonium cerous sulphate solution Ce(NH4)(SO4)4.H2O in which Mr=632.6, and a calibration liquid, a iodine standard series solution (a Potassium Iodate solution KIO3,Mr=214.0).

Description

technical field [0001] The invention relates to the field of medical detection, in particular to reagents, kits, applications and detection methods. Background technique [0002] Iodine is one of the essential trace elements for the human body, known as the "intelligence element". In 1994, urinary iodine was jointly selected by the World Health Organization (WHO), the United Nations Children's Fund (UNICEF) and the International Council for the Control of Iodine Deficiency Diseases (ICCIDD) a biochemical indicator. The total amount of iodine in a healthy adult body is about 30mg (20-50mg), of which 70%-80% exists in the thyroid gland. Iodine is an element necessary to maintain the normal function of the human thyroid gland. However, excessive intake of iodine is also harmful to the human body. Excessive dietary iodine can cause "hyperthyroidism". [0003] There are many methods for the determination of iodine content, and the commonly used methods are: arsenic-cerium cat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31G01N1/34
CPCG01N1/34G01N21/31G01N21/78G01N2021/775
Inventor 王九宏
Owner 王九宏
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