The invention provides a kit for quantitatively detecting UCHL-1, and application thereof, and belongs to the technical field of biological detection. The kit is composed of a detection reagent strip, a calibration product, a quality control product and a two-dimensional code; the detection reagent strip comprises a reagent A and a reagent B; the reagent A is a solution prepared by taking an enzyme-labeled UCHL-1 antibody conjugate as a raw material and fully and uniformly mixing the enzyme-labeled UCHL-1 antibody conjugate with a buffer solution B; and the reagent B is a solution prepared by taking a UCHL-1 antibody magnetic particle conjugate as a raw material and fully and uniformly mixing the UCHL-1 antibody magnetic particle conjugate by using a buffer solution C. In the implementation process, the specific components of the buffer solution B and the buffer solution C and the mass ratio of the components are controlled, so the research on indexes such as appearance, accuracy, blank limit, detection limit, quantification limit, linearity, repeatability, difference between calibration product and quality control product bottles and the like of the prepared kit meets the requirements, the kit is verified to be qualified on a full-automatic chemiluminescence immunoassay analyzer, and the measurement result meets the enterprise standard requirements.