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Protein biomarker for diagnosis of osteoporosis and application thereof

A biomarker and osteoporosis technology, applied in the field of protein biomarkers, can solve the problems of small OP heritability and unclear function of disease genes, and achieve excellent sensitivity and specificity

Active Publication Date: 2019-04-02
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The combination of identified genetic susceptibility loci to explain OP heritability is small, a large number of genetic factors remain to be discovered, and the functions of known disease genes are mostly unclear

Method used

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  • Protein biomarker for diagnosis of osteoporosis and application thereof
  • Protein biomarker for diagnosis of osteoporosis and application thereof
  • Protein biomarker for diagnosis of osteoporosis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening of Dysregulated Proteins in Peripheral Blood Mononuclear Cells of Osteoporosis Patients (9:9)

[0030] 1. After obtaining informed consent, 9 patients with osteoporosis and 9 healthy individuals with bone were collected as normal controls. Fasting peripheral blood samples were collected and placed in sodium citrate anticoagulant tubes.

[0031] 2. Peripheral blood mononuclear cells were obtained by density gradient centrifugation and MACS positive sorting method, and the cell proteome was identified and quantified. Specific steps are as follows:

[0032] (1) Peripheral blood mononuclear cell isolation

[0033] 1. Add 15ml of density gradient separation solution to the leucosep tube, centrifuge at 100g for 1min.

[0034] 2. Pour the collected fresh whole blood into a 50ml centrifuge tube, add an equal amount of PBS and mix well, then transfer to a leucosep tube, and centrifuge at 310g for 20min.

[0035] 3. Aspirate and discard the remaining super...

Embodiment 2

[0080] Example 2: Screening of Dysregulated Proteins in Peripheral Blood Mononuclear Cells of OF Patients (18:18)

[0081] This example includes 18 cases of OF patients and 18 cases of controls without fracture history. Patients and controls were matched for age and sex. Collect peripheral blood, separate blood mononuclear cells, extract total protein, enzyme digestion, identification and quantification, case-control difference analysis, identification of differentially expressed proteins.

[0082] The specific experiment and analysis method flow are the same as those in Example 1, and will not be repeated here. The analysis results showed that there was a significant difference in the expression level of ABI1 protein between the osteoporotic fracture group (OF) and the control group (NF) (P figure 2 (Right panel) shows dysregulation of ABI1 protein expression in peripheral blood mononuclear cells from patients with osteoporotic fractures. It can be seen from the figure that...

Embodiment 3

[0083] Example 3: Verification of protein markers in peripheral blood mononuclear cells of patients with osteoporosis

[0084] 1. Identification of protein markers of osteoporosis

[0085] A total of 27 subjects were included in this example. Among them, 9 cases were individuals with normal bone density (CTRL), 9 cases were patients with osteoporosis (OP), and 9 cases were patients with osteoporotic fractures (OF). Collect peripheral blood, separate blood mononuclear cells, extract total cell protein, mix any 3 samples in the group as a small group, and measure the concentration. The specific experiment and analysis method flow are the same as those in Example 1, and will not be repeated here.

[0086] image 3 It shows that there are significant differences in the expression level of ABI1 protein in peripheral blood mononuclear cells of individuals with normal bone density (CTRL), patients with osteoporosis (OP) and patients with osteoporotic fracture (OF) (P=0.0053). It ...

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Abstract

The invention discloses a protein biomarker for diagnosis of osteoporosis and an application thereof, which belongs to the technical field of biological detection. The invention uses Abl Interactor 1protein as a biomarker for the diagnosis of osteoporosis. The protein biomarker provided by the invention can be used for preparing a diagnostic kit, which has excellent diagnostic value when appliedto blood samples for diagnosis of osteoporosis. By using the protein of the present invention alone, the value of AUC diagnosis can reach 0.76. With a cut-off point of 0.376 ng / ml, the sensitivity andspecificity are 73.3% and 64.3%. By combining CTX and P1NP, the AUC diagnosis can reach 0.78. The protein can be used as a biomarker for the diagnosis of human osteoporosis blood samples, which is helpful for the diagnosis of osteoporosis, the identification and the prevention and treatment of high-risk groups of osteoporotic fractures.

Description

technical field [0001] The invention relates to a protein biomarker for diagnosing osteoporosis and its application, belonging to the technical field of biological detection. Background technique [0002] Osteoporosis (Osteoporosis, OP) is a common chronic disease, and its clinical manifestations are short stature, low back pain, and low-trauma fractures (also known as osteoporotic fractures, OF). OF has a high morbidity rate, with a mortality rate as high as 31%. At present, the prevalence rate of osteoporosis among Chinese residents is as high as 8.8‰. Screening new biomarkers of OP, revealing its molecular pathogenic mechanism, establishing an early diagnosis and prediction model of OP, and developing targeted intervention measures are extremely important strategic measures to control OP. [0003] OP is a complex disease regulated by both environmental and genetic factors, among which genetic factors are an important factor affecting the pathogenesis of OP, accounting f...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 邓飞艳雷署丰周旭
Owner SUZHOU UNIV
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