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Yu Liren polypeptide and its preparation method and application

A technology of Yu Liren and protein separation, applied in the field of polypeptide protein, can solve problems such as waste of resources

Active Publication Date: 2021-05-18
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to modern pharmacological studies, amygdalin has the effect of eliminating phlegm, relieving cough and asthma, plumrenoside has a purging effect, plum kernel oil has a laxative effect, and the protein with a high content is discarded as medicine dregs, resulting in a huge waste of resources

Method used

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  • Yu Liren polypeptide and its preparation method and application
  • Yu Liren polypeptide and its preparation method and application
  • Yu Liren polypeptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of Yu Liren polypeptide is as follows:

[0027] 1) Preparation of Yu Liren Protein Isolate

[0028] Add deionized water to the degreased and pulverized Yu Liren powder, adjust the solid-liquid ratio to 1:12, adjust the pH to 8.0 with 0.5mol / L NaOH solution, stir in a constant temperature water bath at 30°C for 60 minutes, and use Centrifuge at a speed of 4000rpm / min for 20 minutes and take out the supernatant, adjust the pH of the obtained supernatant to 4.5 with 0.5mol / L hydrochloric acid, then centrifuge at 4000rpm / min for 20 minutes to separate the protein, and use Washed three times with deionized water, redissolved with deionized water, adjusted the pH to 7.0 with 0.5 mol / L NaOH, and freeze-dried to obtain the isolated protein of Yu Liren.

[0029] 2) Enzymatic hydrolysis of Yu Liren isolated protein

[0030] Use deionized water to prepare a 5% Yuliren protein solution, boil it for 5 minutes to denature it, put it in a water bath at 50°C, a...

Embodiment 2

[0055] The preparation method of Yu Liren polypeptide is as follows:

[0056] 1) Preparation of Yu Liren Protein Isolate

[0057] Add deionized water to the degreased and pulverized Yu Liren powder, adjust the solid-liquid ratio to 1:12, adjust the pH to 7.5 with 0.5mol / L NaOH solution, stir in a constant temperature water bath at 25°C for 100 minutes, and use The centrifuge was centrifuged at 2500rpm / min for 30 minutes and the supernatant was taken out. The resulting supernatant was adjusted to pH 3.5 with 0.5mol / L hydrochloric acid, then centrifuged at 2500rpm / min for 30 minutes to separate the protein and used Washed three times with deionized water, redissolved with deionized water, adjusted the pH to 6.5 with 0.5 mol / L NaOH, and freeze-dried to obtain Yu Liren protein isolate.

[0058] 2) Enzymatic hydrolysis of Yu Liren isolated protein

[0059] Use deionized water to prepare a 4% Yuliren protein solution, boil it for 4 minutes to denature the protein, put it in a 30°C...

Embodiment 3

[0065] The preparation method of Yu Liren polypeptide is as follows:

[0066] 1) Preparation of Yu Liren Protein Isolate

[0067] Add deionized water to the degreased and pulverized Yu Liren powder, adjust the solid-liquid ratio to 1:12, adjust the pH to 12 with 0.5mol / L NaOH solution, stir in a constant temperature water bath at 75°C for 10 minutes, and use Centrifuge at a speed of 5000rpm / min for 5 minutes and take out the supernatant, adjust the pH of the obtained supernatant to 5.5 with 0.5mol / L hydrochloric acid, then centrifuge at 5000rpm / min for 5 minutes to separate the protein, and use Washed three times with deionized water, redissolved with deionized water, adjusted the pH to 7.5 with 0.5 mol / L NaOH, and freeze-dried to obtain Yu Liren protein isolate.

[0068] 2) Enzymatic hydrolysis of Yu Liren isolated protein

[0069] Use deionized water to prepare a 3% Yuliren protein solution, boil for 3 minutes to denature the protein, put it in a 60°C water bath, adjust th...

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Abstract

The present invention provides a method for preparing Prunus japonicus polypeptide, comprising: 1) separating the protein from Prunus japonicus to obtain the isolated protein of Prunus japonicus; 2) enzymatically hydrolyzing the isolated protein of Prunus japonicus obtained in step 1), to obtain an enzymatic hydrolyzate of Prunus japonicus protein; 3) The Yu Liren protein enzymatic hydrolyzate obtained in step 2) is subjected to in vitro simulated gastrointestinal tract digestion. 4) Separating the product of step 3) in vitro simulated gastrointestinal tract digestion with an ultrafiltration membrane to obtain polypeptides with different molecular weights. The present invention also provides the above-mentioned Yuliren polypeptide and its application. In the present invention, the enzymatic hydrolyzate of Yu Liren protein is digested in vitro to simulate the gastrointestinal tract, and the in vitro simulated gastrointestinal tract is digested by an ultrafiltration membrane to obtain polypeptides with different molecular weights, and the enzymes for α-glucosidase, α-amylase and DPP-IV are obtained. The activity of Yu Liren polypeptide with good inhibitory effect.

Description

technical field [0001] The present invention relates to polypeptide protein, especially Yu Liren polypeptide and its preparation method and application. Background technique [0002] Yu Liren is the dry and mature seed kernel of Rosaceae plants, which is very abundant in my country and is a traditional medicinal and edible raw material in my country. Yu Liren is flat in nature, pungent, bitter, and sweet in taste, and returns to the spleen, large intestine, and small intestine meridian. Yuliren contains amygdalin, yulirenin, fatty oil (more than 50%), organic acids, crude protein, cellulose and other components, which are rich in protein, generally with an average content of about 28%. According to modern pharmacological studies, amygdalin has the effect of eliminating phlegm, relieving cough and asthma, plum glucoside has a purging effect, plum kernel oil has a laxative effect, and the protein with a high content is discarded as medicine dregs, resulting in a great waste o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12P19/14C07K1/34
CPCC07K1/34C12P19/14C12P21/06
Inventor 王身艳李璇薛峰王娟红王艺朱紫昕
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE