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A kind of extraction method of wheat germ peptide

An extraction method and wheat germ technology are applied in the field of extraction of wheat germ peptides, which can solve the problems of complex extraction process, long extraction period and low yield, and achieve the effects of simple extraction process, improved taste and high purity

Active Publication Date: 2021-03-16
HEBEI ZIFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of complex extraction process, long extraction period, high cost and low yield in the existing extraction method of wheat germ peptide, the present invention provides an extraction method of wheat germ peptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting wheat germ peptides, comprising the following process steps:

[0025] a. Weigh 100g of wheat germ meal, add 700g of purified water, stir well, and cook at 95°C for 25 minutes to denature the protein in the germ meal, accelerate the hydrolysis process of protease, and shorten the extraction cycle of wheat germ peptide.

[0026] b. Cool down to 54°C, add 1g of cellulase, add 1g of amylase and 0.5g of glucoamylase after 0.5h, keep the temperature at 54°C, stir and enzymatically hydrolyze for 2.0h; use a centrifuge to desugar and obtain desugared germ The cellulase in it is fermented by Aspergillus niger. The wheat germ meal contains a large amount of cellulose, and most of the protein is wrapped by cellulose, which is not conducive to the contact between protein and protease. The addition of cellulase solves the problem of wheat germ peptide Difficult to extract the problem, degrade cellulose into small molecule monosaccharides, release the protein ...

Embodiment 2

[0032] A method for extracting wheat germ peptides, comprising the following process steps:

[0033] a. Weigh 100g of wheat germ meal, add 800g of purified water, stir well, and cook at 95°C for 25 minutes to denature the protein in the germ meal, accelerate the hydrolysis process of protease, and shorten the extraction cycle of wheat germ peptide.

[0034]b. Cool down to 56°C, add 0.8g of cellulase, add 1.2g of amylase and 0.8g of glucoamylase after 0.5h, keep the temperature at 56°C, stir for 2h for enzymatic hydrolysis; use a centrifuge to desugar to obtain desugar Germ meal; the cellulase in which is fermented by Aspergillus niger, wheat germ meal contains a lot of cellulose, most of the protein is wrapped by cellulose, which is not conducive to the contact between protein and protease, the addition of cellulase solves the problem of wheat germ meal Peptides are difficult to extract, degrade cellulose into small molecular monosaccharides, release the protein in it, make th...

Embodiment 3

[0039] A method for extracting wheat germ peptides, comprising the following process steps:

[0040] a. Weigh 100g of wheat germ meal, add 800g of purified water, stir well, and cook at 90°C for 25 minutes to denature the protein in the germ meal, accelerate the hydrolysis process of protease, and shorten the extraction cycle of wheat germ peptide.

[0041] b. Cool down to 58°C, add 0.5g of cellulase, add 1.5g of amylase and 1g of glucoamylase after 0.5h, keep the temperature at 58°C, stir for 3.0h of enzymatic hydrolysis; use a centrifuge to desugar to obtain desugar Germ meal; the cellulase in which is fermented by Aspergillus niger, wheat germ meal contains a lot of cellulose, most of the protein is wrapped by cellulose, which is not conducive to the contact between protein and protease, the addition of cellulase solves the problem of wheat germ meal Peptides are difficult to extract, degrade cellulose into small molecular monosaccharides, release the protein in it, make th...

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PUM

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Abstract

The invention relates to the technical field of extraction of natural peptide in plants and in particular discloses an extraction method of wheat germ peptide. The extraction method of the wheat germpeptide comprises the following technological steps: a, adding purified water into wheat germ meal and stewing; b, carrying out enzymolysis to obtain desugared germ meal; c, adding sodium citrate; meanwhile, adding neutral proteinase, cellulose proteinase and flavored proteinase; carrying out the enzymolysis for 2.0 to 3.0 h; d, carrying out high-temperature enzyme inactivation; e, separating andpurifying. The extraction method has the advantages that the price of the germ meal as a raw material is low; an extraction process is simple and convenient and no organic solvent is added; the purityof active molecules of the obtained wheat germ peptide is high and the yield of the peptide is high, so that the extraction method has relatively high economic benefits.

Description

technical field [0001] The invention relates to the technical field of extracting natural peptides in plants, in particular to a method for extracting wheat germ peptides. Background technique [0002] Wheat germ is rich in high-quality protein, including 8 kinds of amino acids necessary for the human body, especially the content of lysine accounts for 18.5%, which is 6-7 times higher than that of rice and white flour. Glutathione in wheat germ, some people call it an anticancer factor, has a significant effect on inhibiting cancer. Previously, wheat germ was only used as a by-product of the flour processing industry, mostly used as feed. At present, wheat germ is only roughly processed in China, that is, superfinely pulverized. As a food raw material, it has poor water solubility and poor taste. There is also a part of wheat germ used to make wheat germ oil, and the remaining germ meal is directly thrown away or made into feed, while most of the high-quality protein in wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 张晓东杨广杨子丰
Owner HEBEI ZIFENG BIOTECH
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