Molecular markers of rice sogatella furcifera resistant gene qWBPH3.2 and application of molecular markers

Abstract

The invention relates to a molecular marking method of a rice variety sogatella furcifera resistant gene qWBPH3.2. Rice or breeding material DNA is amplified by the molecular marker C3-2122 primer shown in SEQ ID NO.1 / SEQ ID NO.2, the molecular marker C3-2233 primer shown in SEQ ID NO.3 / SEQ ID NO.4, the molecular marker C3-W2 primer shown in SEQ ID NO.5 / SEQ ID NO.6, or the molecular marker C3-W4 primer: SEQ ID NO.7 / SEQ ID NO.8. If the molecular marker C3-2122 primer can amplify a 202 bp amplified fragment, or the molecular marker C3-2233 primer can amplify a 229 bp amplified fragment, or the 866 bp fragment amplified by the molecular marker C3-W2 primer can be digested into two fragments of 268 bp and 598 bp by a restriction enzyme Hap II, or the 954 bp fragment amplified by the molecularmarker C3-W4 primer can be digested into two fragments of 264 bp and 690 bp by the restriction endonuclease Hind III, it indicates the existence of the rice variety sogatella furcifera resistant geneqWBPH3.2. The resistance level of rice to sogatella furcifera can be predicted with the method, and selection efficiency of the sogatella furcifera resistant line is greatly improved.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and in particular relates to a molecular marker of rice white-backed planthopper-resistant gene qWBPH3.2 and an application thereof. Background technique [0002] Rice is one of the most important food crops in the world, and a sustained and steady increase in rice production is an important guarantee for world food security. However, rice production is threatened by various pests and diseases. Among them, the white-backed planthopper is a seasonal migratory pest of the Homoptera planthopper family. It mainly gathers in the lower part of rice plants and damages rice by sucking nutrients from the phloem. In severe cases, the basal tissue of rice will be necrotic, the plants will fall and die, and the yield will be seriously damaged. The white-backed planthopper not only directly feeds on rice and causes mechanical damage to plants by laying eggs, but also is the main carrier of viral diseases su...

AI Technical Summary

Problems solved by technology

However, due to the high reproduction rate of white-backed planthoppers, the selective pressure of the environment will accelerate the evolution of the population, so breeding practice needs to continuously explore new sources of resistance to white-backed planthoppers, and select newer resistant varieties

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