A special diluent for detecting Streptococcus pneumoniae in urine samples

Abstract

The invention relates to a special diluent for detecting Streptococcus pneumoniae in urine samples. The diluent is composed of five components: borax, 1307Prill, ε-polylysine, C-reactive protein polyclonal antibody, and purified water. The content of each component is as follows: borax as component I content is 50mM ~ 100mM, 1307Prill as component II content is 0.5% ~ 1%, ε‑polylysine as component III content is 0.5% ~ 1%, C‑reaction The content of protein polyclonal antibody as component IV is 10ug / ml-20ug / ml, and the rest is purified water. The diluent mixed with the urine sample at a volume ratio of 1:9 can effectively inhibit the interference of the C-reactive protein in the urine sample on the detection results of Streptococcus pneumoniae, significantly improve the detection rate of Streptococcus pneumoniae, and play an important role in the accurate detection of urine Streptococcus pneumoniae in fluid samples is of great significance and has good clinical application value.

Description

Technical field [0001] The present invention relates to a dedicated diluent of pneumococcal bacteria in urine samples, which can quickly and efficiently and effectively dissociate C-reactive protein (CRP) and pneumoniae C-polysaccharides in urine samples, while inhibiting CRP Biological activity belongs to the field of immunological applications, suitable for immunological assay, immunogefiltration, enzyme-linked immunosorbent experiment, immunological turbidity, chemiluminescence and other immunological experiment methods. Background technique [0002] Pneumonia Streptococcus is the main pathogens caused by bacterial pneumonia. The mortality rate of pneumococcal pneumonia is as high as 30%, depending on the bacterialmia, age, and potential diseases. If the pneumoniae infection can cause bactelatin, meningitis, perfangitis, suppurator, uneven purpura, endocarditis, and / or arthritis. All age groups can be infected, but are more common in children under the age of 5, teens and el...

AI Technical Summary

Problems solved by technology

[0006] Through density gradient centrifugation, gel electrophoresis, western blotting and other technical means, we found that CRP was combined with the C-polysaccharide antigen of Streptococcus pneumoniae in the urine of patients infected with Streptococcus pneumoniae, so we speculated that this combination may be effective for Streptococcus pneumoniae. The detection of S. pneumoniae caused interference, which in turn affected the detection rate of Streptococcus pneumoniae