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Traditional Chinese medicine composition for resisting grouper iridovirus Guangxi strains and application of traditional Chinese medicine composition

An iridescent virus and grouper technology is applied in the field of anti-grouper iridescent virus traditional Chinese medicine compositions, which can solve the problems of large side effects of western medicine, harmful to fish, low efficiency and the like, and achieve the effects of safe toxic side effects and no toxic side effects.

Active Publication Date: 2019-04-12
GUANGXI ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no approved and marketable effective antiviral drug against grouper iridescent virus disease, and the grouper aquaculture industry is in the predicament of no drug available
However, it is common for farmers to blindly use medicines and abuse western medicines in pursuit of high profits. However, western medicines have large side effects and low efficiency. They also cause major food safety problems such as harmful drug residues in fish and serious consequences such as the continuous deterioration of the breeding environment.

Method used

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  • Traditional Chinese medicine composition for resisting grouper iridovirus Guangxi strains and application of traditional Chinese medicine composition
  • Traditional Chinese medicine composition for resisting grouper iridovirus Guangxi strains and application of traditional Chinese medicine composition
  • Traditional Chinese medicine composition for resisting grouper iridovirus Guangxi strains and application of traditional Chinese medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 anti-grouper iridescent virus compound Chinese medicine Qing Ointment

[0030] Weigh the traditional Chinese medicine composition according to the weight ratio of the formula, add water to decoct for 60 minutes, filter with five layers of sterile gauze, and then concentrate the filtrate to a clear paste with a concentration of 1g / mL. The composition of each formula is shown in Table 1.

[0031] The weight proportion of Chinese medicine raw materials of each formula of table 1

[0032] recipe number

Embodiment 2

[0033] Example 2 The inhibitory effect of anti-grouper iridescent virus compound Chinese medicine Qing ointment on grouper iridescent virus

[0034] The GS cells were introduced into a 24-well plate at a ratio of 40,000 / well, cultured at 28°C for 24 hours, and the cells grew into a monolayer. Then, 5 kinds of anti-grouper iridescent virus compound traditional Chinese medicine clear ointments of Example 1 were diluted to different concentrations (10, 5, 1 mg / mL) in the cell culture medium respectively, and then the culture medium containing the medicinal solution and the GIV-Gx-infected GS cells were added to the cells in a 24-well plate and cultured at 28°C; two control groups were set up, with no addition of anti-grouper iridescent virus compound Chinese medicine clear cream and GIV-Gx-infected GS cells were cultured Base was used as control group 1, and the culture medium added only with GIV-Gx-infected GS cells was used as control group 2. After 48 hours, the cells and med...

Embodiment 3

[0036] Example 3 Effect of anti-grouper iridescent virus compound traditional Chinese medicine Qing ointment on the vitality of grouper spleen cells

[0037] GS cells were introduced into 96-well plates at a ratio of 10,000 cells / well, cultured at 28°C for 24 hours, and then the formula ③ corresponding anti-grouper iridescent virus compound traditional Chinese medicine clear ointment was added to the cell culture medium of the 5 groups, so that it could be cured in 5 The concentration in the cell culture medium of the group was 20, 15, 10, 5, 1 mg / mL respectively, and the cell culture medium without anti-grouper iridovirus compound traditional Chinese medicine clear cream was used as a control. The above six groups of cell culture media were incubated with the GS cells in the 96-well plate at 28° C. for 48 hours. In order to detect cell viability, cells in each well were added with 20 μL of WST-8 solution and continued to incubate at 28°C for 4 hours, and then the absorbance a...

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Abstract

The invention discloses a traditional Chinese medicine composition for resisting grouper iridovirus Guangxi strains. The traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials of Guangxi rhizoma curcumae, herba violae and honeysuckle flowers, and is used for resisting the grouper iridovirus Guangxi strains. The traditional Chinese medicine composition is used as an effective action ingredient of medicines for preventing and treating the grouper iridovirus Guangxi strains. Through reasonable compatibility, the situation that the grouper iridovirus Guangxi strains are adsorbed to host cells can be interfered, the grouper iridovirus Guangxi strains can be prevented from penetrating cells, and the grouper iridovirus Guangxi strains can be restrained from duplication and synthesis in the host cells, so that the effect of resisting the grouper iridovirus Guangxi strains can be achieved; and the traditional Chinese medicine composition hasthe characteristics of being high in effective rate, safe and free from toxic and side effects.

Description

technical field [0001] The invention belongs to the field of prevention and treatment of aquatic animal diseases, and in particular relates to an anti-grouper iridescent virus traditional Chinese medicine composition and application thereof. Background technique [0002] my country is a big aquaculture country. Grouper is one of the most valuable mariculture fishes in the southern coastal provinces of my country and Southeast Asian countries. In recent years, the scale of aquaculture has continued to expand. In 2017, the total amount of grouper aquaculture in my country reached more than 130,000 tons. Very high economic value. However, the current outbreak and prevalence of iridescent viruses in grouper has caused huge economic losses and seriously threatened the healthy and sustainable development of grouper aquaculture. Grouper iridescent virus belongs to the genus Ranairidovirus in the family Iridoviridae, and is a highly pathogenic iridescent virus isolated and identifie...

Claims

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Application Information

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IPC IPC(8): A61K36/9066A61P31/20
CPCA61K36/9066A61P31/20A61K36/355A61K36/86A61K2236/331A61K2300/00
Inventor 李鹏飞秦启伟余庆刘明珠肖贺贺吴思婷
Owner GUANGXI ACAD OF SCI
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