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Application of dandelion polysaccharides in preparation of drugs for inhibiting angiogenesis of hepatocellular carcinoma cells

A technology for inhibiting liver cancer and angiogenesis, applied in the field of medicine, can solve the problem of not inhibiting the blood vessels of liver cancer cells, and achieve the effect of inhibiting tumor angiogenesis and broad application prospects

Inactive Publication Date: 2019-04-30
XINXIANG MEDICAL UNIV
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Problems solved by technology

In addition, the known pharmacological effects of dandelion polysaccharides include antibacterial, anti-oxidation, immune regulation, anti-mutation, anti-fatigue and hormone regulation, but it does not have the effect of inhibiting angiogenesis of liver cancer cells

Method used

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  • Application of dandelion polysaccharides in preparation of drugs for inhibiting angiogenesis of hepatocellular carcinoma cells
  • Application of dandelion polysaccharides in preparation of drugs for inhibiting angiogenesis of hepatocellular carcinoma cells
  • Application of dandelion polysaccharides in preparation of drugs for inhibiting angiogenesis of hepatocellular carcinoma cells

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Embodiment 1

[0029] The invention relates to the application of dandelion polysaccharide in the preparation of drugs for inhibiting angiogenesis of liver cancer cells. The present invention also provides that the liver cancer includes human liver cancer HepG2 cells, human umbilical vein endothelial cells HUVEC, and mouse liver cancer cells H22. The drug for inhibiting angiogenesis of liver cancer cells can inhibit the migration of human umbilical vein endothelial cells HUVEC. The drug for inhibiting angiogenesis of liver cancer cells can inhibit the formation of human umbilical vein endothelial cells HUVEC blood vessel. The drug for inhibiting angiogenesis of liver cancer cells can inhibit the formation of blood vessels in subcutaneous tumor tissue of mouse liver cancer cells H22. The drug for inhibiting angiogenesis of liver cancer cells can inhibit the expression of VEGF and VEGFR genes in human liver cancer HepG2 cells. The drug for inhibiting angiogenesis of liver cancer cells can in...

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Abstract

The invention belongs to an application of dandelion polysaccharides in preparation of drugs for inhibiting angiogenesis of hepatocellular carcinoma cells; the effects of the dandelion polysaccharideson angiogenesis are observed by scratch tests, eanaliculization tests and chick embryo chorioallantoic membrane angiogenesis tests, and the effects of the dandelion polysaccharides on the angiogenesis of subcutaneous tumors of H22 hepatocellular carcinoma cell-bearing mice are studied by immunohistochemistry; qPCR, Western blot and ELISA are used for detecting the expression of angiogenesis-related factor mRNA and proteins at cellular and animal levels respectively; the dandelion polysaccharides can be effectively proved to play an anti-tumor role by inhibiting tumor angiogenesis, and tumor angiogenesis can be inhibited in vivo and in vitro by down-regulating the expression of tumor cells VEGF and VEGF-2. The dandelion polysaccharides have the advantage of effectively inhibiting tumor angiogenesis to achieve anti-tumor effect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of dandelion polysaccharide in the preparation of drugs for inhibiting angiogenesis of liver cancer cells. Background technique [0002] Angiogenesis, the term angiogenesis was coined in a 1900 paper discussing the growth of the adrenal gland, by Rudolf Sterner, published in Johns Hopkins Hospital Reports. "angio" refers to blood vessels, and "genesis" refers to origin; angiogenesis is a key factor in the formation of neovascularization in tumors. Angiogenesis includes the following steps: activation of small vessel endothelial cells, degradation of extracellular matrix, and migration of cells in the matrix , proliferation, the endothelial cell assembly is a hollow tube, and the tube eventually anastomoses to form a new capillary. Recently, a similar process has been proved by animal models of tumor experiments; the effect of angiogenesis on human tu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P1/16A61P35/00A61K36/288
CPCA61K31/715A61K36/288A61P1/16A61P35/00
Inventor 李健任峰杨赟罗艳艳吴凯旋冯思佳王阳林
Owner XINXIANG MEDICAL UNIV
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