Method for enhancing rhodamine B isothiocyanate fluorescence intensity through silver nanoparticle aggregation

A technology of silver nanoparticles and isothiocyanate, which is applied in fluorescence/phosphorescence, material analysis through optical means, and measurement devices, can solve the problems of complex synthesis process and achieve simple preparation process, good reproducibility, and reaction The effect of mild conditions

Active Publication Date: 2019-04-30
SOUTH CHINA UNIV OF TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, most experimental methods use metal thin films as substrates, instead of using them for imaging in vivo, and use metal nanoparticles as substrates, which need to be coated with a layer of silica or polyelectrolyte to control the distance, and the synthesis process is complicated.

Method used

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  • Method for enhancing rhodamine B isothiocyanate fluorescence intensity through silver nanoparticle aggregation
  • Method for enhancing rhodamine B isothiocyanate fluorescence intensity through silver nanoparticle aggregation
  • Method for enhancing rhodamine B isothiocyanate fluorescence intensity through silver nanoparticle aggregation

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Embodiment

[0033] (1) Preparation of AgNPs

[0034] 1) Prepare silver nitrate solution with a concentration of 59.4mmol / L, sodium citrate solution with a concentration of 34mmol / L, ascorbic acid solution with a concentration of 0.2mol / L, and sodium chloride solution with a concentration of 0.2mol / L ;

[0035] 2) Heat and stir 49 mL of deionized water at 120°C and 350 rpm, and add 1 mL of the sodium citrate solution, 100 μL of the ascorbic acid solution and 20 μL of the sodium chloride solution when boiling, Continue heating and stirring at 120°C and 350 rpm for 5 minutes;

[0036] 3) Add 0.25 mL of the silver nitrate solution at 120 °C and 350 rpm, and the color of the solution changes rapidly from colorless to yellow, indicating the formation of AgNPs. Continue to stir and keep warm for 1 hour to complete the reaction, turn off the heating, continue to stir at a speed of 350 rpm, and turn off the stirring when it is naturally cooled to room temperature to obtain the AgNPs solution.

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Abstract

The invention discloses a method for enhancing rhodamine B isothiocyanate fluorescence intensity through silver nanoparticle aggregation. The method comprises the following steps: (1) preparing silvernanoparticles; (2) modifying the surfaces of the silver nanoparticles with rhodamine B isothiocyanate to obtain silver nanoparticles with the surfaces modified with rhodamine B isothiocyanate; and (3) adding a connecting agent, and aggregating the silver nanoparticles with the surface modified by rhodamine B isothiocyanate to enhance the fluorescence intensity of the rhodamine B isothiocyanate. According to the method, a hot spot generated by aggregation and coupling of precious metal nanoparticles is mainly utilized, so that a local electric field among the nanoparticles is remarkably enhanced, the fluorescence emission efficiency of rhodamine B isothiocyanate on the surface of the nanoparticles is improved, and rhodamine B isothiocyanate fluorescence quenched by silver nanoparticles under monodispersion is recovered and even enhanced. The method is obvious in fluorescence enhancement, the sample can be dispersed in a water phase, and fluorescence change is obvious before and after aggregation.

Description

technical field [0001] The invention relates to the field of synthesis and application of nanometer materials, in particular to a method for enhancing the fluorescence intensity of rhodamine B isothiocyanate, and the technology can be used for biological imaging, analysis and detection, and the like. Background technique [0002] Noble metal nanoparticles, such as gold and silver nanoparticles, have been widely used in many fields, such as biosensing, cell imaging and nonlinear optics, due to their unique optical properties - surface plasmon resonance (SPR) Wait. Metal nanoparticles interact with incident light, causing surface free electrons to oscillate collectively to produce a surface plasmon resonance effect. The surface plasmon resonance absorption peak position is sensitive to environment, size, and particle shape, and can also be changed by coupling with nanoparticle aggregation. When the nanoparticles aggregate, the surface plasmon coupling leads to a red shift of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/643G01N2021/6432
Inventor 徐清华张鼎丰李爽
Owner SOUTH CHINA UNIV OF TECH
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