Boehmeria nivea leaf back velutinous main effect QTL and closely linked molecular marker and application thereof

A ramie leaf and marking technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of reduced feeding performance and poor palatability of ramie, and achieve the effect of good technical support

Pending Publication Date: 2019-05-03
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The felt hair on the back of ramie leaves is mainly cuticle hair fiber, which exists in a large amount in c

Method used

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  • Boehmeria nivea leaf back velutinous main effect QTL and closely linked molecular marker and application thereof
  • Boehmeria nivea leaf back velutinous main effect QTL and closely linked molecular marker and application thereof
  • Boehmeria nivea leaf back velutinous main effect QTL and closely linked molecular marker and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The acquisition of the SNP marker of ramie leaf dorsal felt wool is as follows:

[0069] 110 single-plant RaF2 populations were obtained by crossing the parent Zhongsizhu 1 with Qingye Ramie, and the 110 RaF2 populations and the transcriptomes of the two parents were sequenced;

[0070] A high-density genetic map containing 1085 molecular markers was constructed by Jionmap4.0 software, and the 1085 markers were evenly distributed on 14 linkage groups, with an average marker spacing of 1.95cM;

[0071] According to the RaF2 phenotype data, combined with the high-density linkage map, the traits were detected by MapQTL (version: 6.0) software, and the 1085 SNP sites on the integrated genetic map were compared with 2 by using plink (version: 1.07) software through GWAS. The association analysis of a single SNP locus is carried out for each trait, and the results are as follows figure 1 shown.

[0072] figure 1 , the upper half of the figure shows the LOD values ​​obtaine...

Embodiment 2

[0099] Based on the SNP marker of ramie leaf dorsal hair on chromosome 7 of ramie, the SSR and INDEL markers of ramie leaf dorsal hair were developed based on the scaffold sequence of chromosome 7, and then the SSR and INDEL primers were designed with PRIMER5 and DNAMAN. 16 pairs of SSR primers and 7 pairs of INDEL primers were synthesized by a biological company.

[0100] The genotype identification of 110 individual RaF2 populations obtained from crossing Sizhu 1 and Qingye Ramie in the parent, the process includes the following steps:

[0101] 1) DNA extraction work;

[0102] 2) Use 16 pairs of synthetic SSR primers and 7 pairs of INDEL primers to perform PCR amplification on the genome of each individual plant, and then analyze the bands by electrophoresis for genotype analysis. The genotype consistent with Zhongsizhu No. 1 is marked as "Z", and the same genotype as Qingye Ramie is marked as "Q". If a single plant contains both Zhongsizhu 1 and Qingye Ramie genotypes, it ...

Embodiment 3

[0115] Based on the SNP marker of ramie leaf dorsal hair on chromosome 13 of ramie, SSR and INDEL markers were developed based on the scaffold sequence of chromosome 13, and then primers for SSR and INDEL were designed with PRIMER5 and DNAMAN. 17 pairs of SSR primers were synthesized by a biological company.

[0116] The genotype identification of 110 individual RaF2 populations obtained from crossing Sizhu 1 and Qingye Ramie in the parent, the process includes the following steps:

[0117] 1) DNA extraction work;

[0118] 2) Using SSR primer 17 to perform PCR amplification on the genome of each individual plant, and then analyze the bands by electrophoresis for genotype analysis. The same genotype of Zhongsizhu No. 1 is marked as "Z", and the same genotype of Qingye Ramie is marked as "Q". If a single plant contains both Zhongsizhu 1 and Qingye Ramie genotypes, then The heterozygous genotype is marked as "H".

[0119] Some electrophoresis results such as Figure 5-7 shown...

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Abstract

The invention relates to the field of boehmeria nivea leaf back velutinous molecular biology, in particular to a boehmeria nivea leaf back velutinous main effect QTL and a closely linked molecular marker and application thereof. The invention provides a boehmeria nivea leaf back velutinous SNP marker located on a No. 7 chromosome and a No. 13 chromosome and an application of the boehmeria nivea leaf back velutinous SNP marker. The boehmeria nivea leaf back velutinous SNP marker provides theory support for detection of boehmeria nivea leaf back velutinous properties and new variety breeding andpopulation heredity diversity. The invention also provides a boehmeria nivea leaf back velutinous pertinent SSR sequence and an application thereof, and the SSR sequence provides favorable technicalsupport for boehmeria nivea leaf back velutinous detection.

Description

technical field [0001] The invention relates to the field of molecular biology of ramie leaf dorsal hairs, in particular to the main effect QTL of ramie leaf dorsal hairs and its closely linked molecular markers and applications. Background technique [0002] Ramie is one of the important fiber crops, and ramie leaves are high in protein and rich in nutrients, and are excellent forage crops. Felt hairs on the back of ramie leaves are mainly epidermal hair fibers, which exist in large quantities in cultivated ramie species, resulting in poor palatability of ramie and significantly reducing its feeding performance. Therefore, searching for the genes related to the velvet hair will provide a good theoretical basis for the improvement of this variety. [0003] In view of this, the present invention is proposed. Contents of the invention [0004] The first object of the present invention is to provide the SNP marker of ramie leaf dorsal wool and its application, which provide...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 刘头明王延周曾铮
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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