Rated micro-fluidic chip for sorting fetal nucleated red blood cells based on double-antibody process, and sorting method based thereon

Abstract

The invention discloses a micro-fluidic chip for sorting fetal nucleated red blood cells based on a double-antibody process. Independent cavities formed by substrates and cover sheet are a sample cell, a buffer solution cell, a first waste liquid cell, a second waste liquid cell, a first capture area and a second capture area respectively, substrates and cover sheets form independent channels, oneends of three channels are respectively communicated with the sample cell, the buffer solution cell and one end of the first capture area, the other ends of the three channels are communicated at a proximal intersection, another three channels are respectively communicated with the first waste liquid cell, one end of the second capture area and the other end of the first capture area, the other ends of the another three channels are communicated at a distal intersection, and the other end of the second capture area is communicated with the second waste liquid cell through one channel. Substances to be detected are sorted by immunomagnetic beads in the first capture area, and an antibody embedded in the second capture area is used to combine and recognize the substances to be detected, sothe sensitivity is improved, and the fetal nucleated red blood cells can be efficiently captured.

Description

technical field [0001] The invention relates to microfluidic chip technology, in particular to a microfluidic chip for sorting fetal nucleated red blood cells and a method for using the same. Background technique [0002] Down syndrome, also known as trisomy 21, is one of the earliest chromosomal diseases discovered. Most of the affected children died in early pregnancy and miscarried. The survivors had obvious congenital mental retardation, special facial features, and multiple organ hypoplasia. It brings heavy mental and economic burdens to the family and society, and affects the birth quality of our country's population. It is one of the routine items for inspection during pregnancy, and it is important to diagnose Down's fetuses in the early and middle trimesters and terminate the pregnancy in time. The main measures to prevent the birth of sick children. [0003] At present, the prenatal diagnosis of Down's syndrome is still based on invasive sampling combined with chr...

AI Technical Summary

Problems solved by technology

Density gradient centrifugation micromanipulation requires effective labeling and identification of target cells. Commonly used methods include Wright's staining, Kl ei hauer acid-fast staining, or immunohistochemistry, while FACS and MACS need to use fluorescein or magnetic beads to label specific cells. However, the equipment is expensive, the amount of cells used is large, and the operation is cumbersome, so it is difficult to be widely promoted in laboratories and hospitals.

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