Method for inspecting aluminium ion based on aluminium ion induced phosphorescent copper nano-cluster gathering enhanced fluorescence and application of method

A technology of copper nano-clusters and enhanced fluorescence, applied in the field of biosensing, can solve the problems of low detection limit, high price, complicated operation, etc., and achieve the effects of fast response, reduced competition, and simple synthesis

Active Publication Date: 2019-05-07
NANJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In these methods, there are generally disadvantages such as low detection limit, high price, complicated operation, etc.

Method used

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  • Method for inspecting aluminium ion based on aluminium ion induced phosphorescent copper nano-cluster gathering enhanced fluorescence and application of method
  • Method for inspecting aluminium ion based on aluminium ion induced phosphorescent copper nano-cluster gathering enhanced fluorescence and application of method
  • Method for inspecting aluminium ion based on aluminium ion induced phosphorescent copper nano-cluster gathering enhanced fluorescence and application of method

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Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1: Get DMF and deionized water configuration 5mL mixed solution in 20mL glass bottle, DMF / H 2 O volume ratio is 2:1. Prepare 0.025M glutathione solution, add 200 μL of glutathione solution to the above mixed solution, shake gently until uniform, and the concentration of GSH in the mixed solution is 1 mM. Then configure 1M copper sulfate solution, take 5 μL of copper sulfate solution into the glass bottle, so that the concentration of copper ions in the mixed solution is 1000 μM. Put the glass bottle into a shaking box at 20°C, stir and shake at a slow speed for 1 minute to form phosphorescent copper nanoclusters. The prepared copper nanoclusters were measured by a fluorescence spectrophotometer to draw a fluorescence curve, such as figure 1 .

Embodiment 2

[0058] Embodiment 2: Get DMF and deionized water configuration 5mL mixed solution in 20mL glass bottle, DMF / H 2 O volume ratio is 2:1. Prepare 0.05M glutathione solution, add 200 μL of glutathione solution to the above mixed solution, shake gently until uniform, and the concentration of GSH in the mixed solution is 2 mM. Then configure 1M copper sulfate solution, take 5 μL of copper sulfate solution into the glass bottle, so that the concentration of copper ions in the mixed solution is 1000 μM. Put the glass bottle into a shaking box at 20°C, stir and shake at a slow speed for 1 minute to form phosphorescent copper nanoclusters. The prepared copper nanoclusters were measured by a fluorescence spectrophotometer to draw a fluorescence curve, such as figure 2 .

Embodiment 3

[0059] Embodiment 3: get DMF and deionized water configuration 5mL mixed solution in 20mL glass bottle, DMF / H 2 O volume ratio is 2:1. Prepare 0.1M glutathione solution, add 200 μL of glutathione solution to the above mixed solution, shake gently until uniform, and the concentration of GSH in the mixed solution is 4 mM. Then configure 1M copper sulfate solution, take 5 μL of copper sulfate solution into the glass bottle, so that the concentration of copper ions in the mixed solution is 1000 μM. Put the glass bottle into a shaking box at 20°C, stir and shake at a slow speed for 1 minute to form phosphorescent copper nanoclusters. The prepared copper nanoclusters were measured by a fluorescence spectrophotometer to draw a fluorescence curve, such as image 3 .

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Abstract

The invention relates to a method for inspecting an aluminium ion based on aluminium ion induced phosphorescent copper nano-cluster gathering enhanced fluorescence and application of the method, and belongs to the technical field of nano-biology sensing. In room temperature, glutathione and copper sulfate are added to a mixed solvent of DMF and deionized water to prepare copper nano-cluster (Cu NCs) with phosphorescent characteristics. The aluminium ion (Al3+) can effectively enhance the fluorescence of the Cu NCs, and the fluorescence of Cu Ncs / Al3+ system is reduced due to the fact that pyrophosphate and the aluminium ion have high affinity. The co-incubation solution of alkaline phosphatase and the pyrophosphate is added into the Cu Ncs / Al3+ system, and due to the fact that the alkalinephosphatase can hydrolyze the pyrophosphate, and the competition function of the pyrophosphate on Al3+ in Cu Ncs / Al3+ system is effectively reduced. The quantitative detection on the alkaline phosphatase can be realized through variation of the fluorescence intensity.

Description

technical field [0001] The invention relates to a method for detecting aluminum ions based on aluminum ion-induced aggregation of phosphorescent copper nano-clusters to enhance fluorescence and an application thereof, belonging to the technical field of biosensing. Background technique [0002] Metal nanoclusters are composed of several to dozens of metal atoms, and the size is close to the electron Fermi wavelength. It is a new type of fluorescent nanomaterial. Due to the ultra-small size of metal nanoclusters, it has excellent size effect, excellent photophysical properties and catalytic effect. From biological probes to catalysts, LEDs to photovoltaic cells, metal clusters have been widely studied and applied in many fields. Among them, metal copper nanoclusters have higher reactivity and higher correlation with molecular proteins and enzymes. In recent years, metal nanoclusters have attracted widespread attention due to their unique chemical and physical properties, an...

Claims

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Application Information

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IPC IPC(8): G01N21/64B82Y40/00B82Y15/00B22F9/24B22F1/00
Inventor 刘金华慈乔乔常进张承武李林张倩晨邹昌鹏
Owner NANJING UNIV OF TECH
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