Glycated hemoglobin extraction kit and extraction method

A technology of glycosylated hemoglobin and extraction method, which is applied in the field of glycosylated hemoglobin extraction kit and extraction, can solve the problems of high equipment requirements, unfavorable scientific research, glycosylated hemoglobin cannot be stored for a long time, and achieve the effect of uniform components

Active Publication Date: 2022-03-29
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above three technologies have high requirements on equipment, and the extracted glycosylated hemoglobin cannot be stored for a long time after recovery, which is not conducive to scientific research

Method used

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  • Glycated hemoglobin extraction kit and extraction method
  • Glycated hemoglobin extraction kit and extraction method
  • Glycated hemoglobin extraction kit and extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The glycosylated hemoglobin extraction kit of the present invention mainly consists of cleaning solution A, cleaning solution B, eluent and storage solution, wherein:

[0038] The composition and content of cleaning solution A are as follows:

[0039]

[0040] The composition and content of cleaning solution B are as follows:

[0041]

[0042] The composition and content of the eluent are as follows:

[0043]

[0044] The composition and content of the storage solution are as follows:

[0045]

Embodiment 2

[0047] The glycosylated hemoglobin extraction kit of the present invention mainly consists of cleaning solution A, cleaning solution B, eluent and storage solution, wherein:

[0048] The composition and content of cleaning solution A are as follows:

[0049]

[0050] The composition and content of cleaning solution B are as follows:

[0051]

[0052] The composition and content of the eluent are as follows:

[0053]

[0054] The composition and content of the storage solution are as follows:

[0055]

Embodiment 3

[0057] The glycosylated hemoglobin extraction kit of the present invention mainly consists of cleaning solution A, cleaning solution B, eluent and storage solution, wherein:

[0058] The composition and content of cleaning solution A are as follows:

[0059]

[0060]

[0061] The composition and content of cleaning solution B are as follows:

[0062]

[0063] The composition and content of the eluent are as follows:

[0064]

[0065] The composition and content of the storage solution are as follows:

[0066]

[0067] The kits of the above three embodiments are used to extract the glycated hemoglobin, and the extraction method can adopt the following steps:

[0068] Step 1: Place the collected blood at room temperature for half an hour;

[0069] Step 2: Add the cleaning solution A to the collected plasma for treatment at 37 degrees, then add the cleaning solution B, mix upside down, centrifuge and discard the supernatant, repeat the operation until the supern...

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PUM

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Abstract

The invention discloses a glycosylated hemoglobin extraction kit and an extraction method, which relate to the field of biotechnology. The extraction kit is composed of cleaning solution A, cleaning solution B, eluent and storage solution, wherein: the components of the cleaning solution A are as follows: buffer solution pH7.0, sodium chloride, potassium chloride, sodium azide; The composition of the cleaning liquid B is as follows: HEPS Buffer, lithium chloride, magnesium sulfate, TCEP; the composition of the eluent is as follows: ammonium sulfate, ammonium chloride, triton, ethylene glycol; the storage liquid The ingredients are as follows: glycerin, TCEP, stabilizer, sodium azide. The invention has low requirements on operating equipment, and the extracted glycosylated hemoglobin can be preserved for a long time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an extraction kit and an extraction method for glycosylated hemoglobin. Background technique [0002] There are three forms of hemoglobin in normal blood: HbA, HbF, and HbA2. Generally, HbA is the main component in adult red blood cells. By separating hemoglobin using chromatography, three glycated hemoglobins can be separated: HbAla, HbAlb, and HbAlc, which together make up glycated hemoglobin. Glycated hemoglobin is produced by the combination of hemoglobin and blood sugar in red blood cells in human blood. The higher the value of glycosylated hemoglobin, the more hemoglobin and blood sugar are combined, and the diabetes becomes more serious. Red blood cells in the human body can generally last for 120 days, and the level of glycosylated hemoglobin generally reflects the average blood sugar level of the 120 days before the test. [0003] At present, the extraction methods for gly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/805C07K1/14
Inventor 李静李建营甘宜梧罗维晓李志明
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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